Publications(
CL-0112
$ 500.00
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1×10^6Cells/Vial×2Vials
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Product Introduction
General information
| Cell Name | HMy2.CIR |
| Synonyms | Hmy.2 CIR;HMy2.CIR;C1R |
| Cellosaurus Accession | CVCL_3714 |
| Organism | Human |
| Growth Properties | Suspension |
| Morphology | Lymphoblast-like |
| Tissue | B lymphoblast |
| Age | 33Y |
| Sex | Female |
Handling information
| Instructions | 1. Check all containers for leakage or breakage. 2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation. 3. This cell line is a suspension cell line. Harvest the cell suspension by centrifugation; do not discard the culture medium directly. |
| Complete Medium | IMDM [PM150510]+10% Nutrient+1% Supplement |
| Incubation Atmosphere | Air, 95%; CO₂, 5% |
| Temperature | 37℃ |
| Subcultivation Ratio | 3×10⁵-1×10⁶ cells/mL |
| Medium Renewal | 2 to 3 times per week |
| Subculturing Procedure | Cultures can be maintained by either supplementing with fresh medium or exchanging medium via centrifugation. The recommended centrifugation conditions are 1200 rpm (approximately 250 × g) for 3 minutes. |
| Freeze Medium | General Freezing Medium [PB180436] |
| Storage Conditions | For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150°C to −196°C. Storage at −80°C is restricted to short-term interim use only. |
Reference materials
| Background | HMy2.CIR cells were derived from Hmy.2B, a fast‑growing mutant of the ARH‑77 cell line, by γ‑irradiation followed by selection for loss of HLA class I antigen expression. HMy2.CIR cells do not express products of the HLA‑A and HLA‑B loci, but express low levels of HLA‑Cw4. This cell line is suitable as a transfection host for class I major histocompatibility complex (MHC) genes. ARH‑77 cells have been reported to be positive for Epstein‑Barr nuclear antigen (EBNA⁺) and Epstein‑Barr virus capsid antigen (EBVCA⁺). As HMy2.CIR cells are derived from Hmy.2B, a fast‑growing mutant of ARH‑77, they are speculated to also be EBNA⁺. |
| Biosafety Level | BSL-2 |
Documents
MSDSPublications
hIgDFc-Ig inhibits B cell function by regulating the BCR-Syk-Btk-NF-κB signalling pathway in mice with collagen-induced arthritis
Journal: Pharmacological Research (2021) IF: 9.3
DOI: 10.1016/j.phrs.2021.105873
Product Cited: HMy2.CIR Cells Line
LINC00857 contributes to proliferation and lymphomagenesis by regulating miR-370-3p/CBX3 axis in diffuse large B-cell lymphoma
Journal: Carcinogenesis (2021) IF: 4.7
Product Cited: HMy2.CIR Cells Line
