HT-29

Name: HT-29
Brand: Procell
SKU: CL-0118
Price: 420.0 USD
Availability: InStock

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Cat. No.: CL-0118

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CL-0118

$ 420.00

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1×10^6Cells/Vial×2Vials

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Product Introduction Documents Publications

Product Introduction

General information

Cell Name	HT-29
Synonyms	HT 29;HT29
Cellosaurus Accession	CVCL_0320
Organism	Human
Growth Properties	Adherent
Morphology	Epithelial-like
Disease	colorectal cancer cells
Tissue	Colon
Age	44Y
Sex	Female

Handling information

Instructions	1. Check all containers for leakage or breakage. 2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation.
Complete Medium	McCoy's 5A [PM150710]+10% Nutrient+1% Supplement
Incubation Atmosphere	Air, 95%; CO₂, 5%
Temperature	37℃
Subcultivation Ratio	1:2-1:4
Medium Renewal	2 to 3 times per week
Dissociation Duration	3-5 min
Subculturing Procedure	1. Remove the culture medium from the T25 cell culture flask. 2. Add approximately 2 mL of PBS. Gently tilt the flask side to side until the PBS covers the entire bottom, then aspirate and discard the PBS. 3. Add 1 mL of 0.25% trypsin solution (containing EDTA). Gently tilt the flask side to side until the trypsin solution covers the entire bottom of the flask. 4. Incubate the cells at 37°C. Observe the cells under an inverted microscope and terminate digestion once the cells round up and detach. To avoid clumping, do not agitate the cells by tapping or shaking the flask during detachment. 5. Add 3 mL of complete culture medium to terminate digestion and disperse into a single cell suspension. 6. Collect the cell suspension and centrifuge at 1200 rpm (approximately 250 ×g) for 3 minutes. Carefully aspirate and discard the supernatant. 7. Add fresh complete culture medium, pipette gently several times to resuspend the cells, and seed them at the appropriate ratio into a new culture flask. Loosen the cap or use a vented cap for incubation.
Freeze Medium	General Freezing Medium [PB180436]
Storage Conditions	For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150°C to −196°C. Storage at −80°C is restricted to short-term interim use only.

Reference materials

Background	HT-29 cells were established in 1964 by J. Fogh from a primary tumor via explant culture in F12 medium supplemented with 15% fetal bovine serum (FBS). The cell line is currently maintained in McCoy's 5A medium with the same serum concentration. HT-29 cells are tumorigenic in both nude mice and steroid-treated hamsters, and synthesize IgA, CEA, TGF-β-binding proteins, and mucins.
Tumorigenic	Yes, in nude mice; forms well differentiated adenocarcinoma consistent with colonic primary (grade I); tumors also form in steroid treated hamsters.
Receptor Expression	human adrenergic alpha2A, urokinase receptor (u-PAR), vitamin D (moderate expression), urokinase receptor (u-PAR); vitamin D (moderate expression), human adrenergic alpha2A
Gene Expression	secretory component of IgA; carcinoembryonic antigen (CEA); transforming growth factor beta binding protein; mucin, myc+; ras+; myb+; fos+; sis+; p53+; abl -; ros -; src -, Blood Type A; Rh+; HLA A1, A3, B12, B17, Cw5, HT-29 cells are negative for CD4, but there is cell surface expression of galactose ceramide (a possible alternative receptor for HIV).
Biosafety Level	BSL-1