HT-55
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Cat. No.: CL-1192
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CL-1192
$ 600.00
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1×10^6Cells/Vial×2Vials
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Product Introduction
General information
| Cell Name | HT-55 |
| Synonyms | HT55 |
| Cellosaurus Accession | CVCL_1294 |
| Organism | Human |
| Growth Properties | Adherent |
| Morphology | Epithelial-like |
| Tissue | Rectum |
| Age | 54Y |
| Sex | Female |
Handling information
| Instructions | 1. Check all containers for leakage or breakage. 2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation. 3. Cells grow in island-like clusters; cell density is relatively high after dissociation. 4. This cell line requires stable culture conditions; minimize movement during culture. 5. This cell line is relatively refractory to dissociation, with a dissociation time of 5–8 minutes, which may be appropriately extended with prolonged culture duration. 6. Complete dissociation into single cells is not required; small clusters of 3–5 cells are normal. 7. Cells can be cryopreserved or subcultured for expansion at 80% confluence; subculture can also be performed at around 50% confluence. 8. Floating dead cells or secretions may appear during culture and can be removed by medium exchange. |
| Complete Medium | DMEM [PM150210]+10% Nutrient+1% Supplement |
| Incubation Atmosphere | Air, 95%; CO₂, 5% |
| Temperature | 37℃ |
| Subcultivation Ratio | 1:2-1:4 |
| Medium Renewal | 2 to 3 times per week |
| Dissociation Duration | 5-8 min |
| Subculturing Procedure | 1. Remove the culture medium from the T25 cell culture flask. 2. Add approximately 2 mL of PBS. Gently tilt the flask side to side until the PBS covers the entire bottom, then aspirate and discard the PBS. 3. Add 1 mL of 0.25% trypsin solution (containing EDTA). Gently tilt the flask side to side until the trypsin solution covers the entire bottom of the flask. 4. Incubate the cells at 37°C. Observe the cells under an inverted microscope and terminate digestion once the cells round up and detach. To avoid clumping, do not agitate the cells by tapping or shaking the flask during detachment. 5. Add 3 mL of complete culture medium to terminate digestion and disperse into a single cell suspension. 6. Collect the cell suspension and centrifuge at 1200 rpm (approximately 250 ×g) for 3 minutes. Carefully aspirate and discard the supernatant. 7. Add fresh complete culture medium, pipette gently several times to resuspend the cells, and seed them at the appropriate ratio into a new culture flask. Loosen the cap or use a vented cap for incubation. |
| Freeze Medium | 90% FBS+10% DMSO |
| Storage Conditions | For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150°C to −196°C. Storage at −80°C is restricted to short-term interim use only. |
Reference materials
| Background | The HT55 cell line is an epithelial cell line derived from the primary tumor of a 54-year-old female patient with rectal adenocarcinoma. The cells grow in an island-like pattern and reach a maximum confluence of approximately 80%. |
| Biosafety Level | BSL-1 |
MSDS
