hTERT-HPNE

Name: hTERT-HPNE
Brand: Procell
SKU: CL-0921
Price: 600.0 USD
Availability: InStock

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Cat. No.: CL-0921

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CL-0921

$ 600.00

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1×10^6Cells/Vial×2Vials

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Product Introduction Documents Publications

Product Introduction

General information

Cell Name	hTERT-HPNE
Synonyms	hTERT-Human Pancreatic Nestin-Expressing cells
Cellosaurus Accession	CVCL_C466
Organism	Human
Growth Properties	Adherent
Morphology	Epithelial-like
Tissue	Pancreas; Pancreatic duct
Age	52Y
Sex	Male

Handling information

Instructions	1. Check all containers for leakage or breakage. 2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation. 3. This cell line occupies a large surface area upon attachment; excessively high subcultivation split ratios are not recommended.
Complete Medium	DMEM [PM150210]+10% Nutrient+1% Supplement
Incubation Atmosphere	Air, 95%; CO₂, 5%
Temperature	37℃
Subcultivation Ratio	1:2-1:4
Medium Renewal	2 to 3 times per week
Dissociation Duration	2-3 min
Subculturing Procedure	1. Remove the culture medium from the T25 cell culture flask. 2. Add approximately 2 mL of PBS. Gently tilt the flask side to side until the PBS covers the entire bottom, then aspirate and discard the PBS. 3. Add 1 mL of 0.25% trypsin solution (containing EDTA). Gently tilt the flask side to side until the trypsin solution covers the entire bottom of the flask. 4. Incubate the cells at 37°C. Observe the cells under an inverted microscope and terminate digestion once the cells round up and detach. To avoid clumping, do not agitate the cells by tapping or shaking the flask during detachment. 5. Add 3 mL of complete culture medium to terminate digestion and disperse into a single cell suspension. 6. Collect the cell suspension and centrifuge at 1200 rpm (approximately 250 ×g) for 3 minutes. Carefully aspirate and discard the supernatant. 7. Add fresh complete culture medium, pipette gently several times to resuspend the cells, and seed them at the appropriate ratio into a new culture flask. Loosen the cap or use a vented cap for incubation.
Freeze Medium	90% FBS+10% DMSO
Storage Conditions	For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150°C to −196°C. Storage at −80°C is restricted to short-term interim use only.

Reference materials

Background

The hTERT-HPNE cell line was established from pancreatic tissue of a 52-year-old male patient and immortalized by transduction with the retroviral expression vector pBABEpuro carrying the hTERT gene. Following transduction, the cells exhibited positive telomerase activity, did not undergo senescence, and continued to proliferate beyond 150 population doublings. hTERT-HPNE cells display features characteristic of intermediate cells formed during acinar-to-ductal metaplasia, including an undifferentiated phenotype, expression of nestin, and evidence of active Notch signaling.

Biosafety Level

BSL-2