HuT 78

Name: HuT 78
Brand: Procell
SKU: CL-0364
Price: 420.0 USD
Availability: InStock

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Cat. No.: CL-0364

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1×10^6Cells/Vial×2Vials

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General information

Handling information

Instructions	1. Check all containers for leakage or breakage. 2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation. 3. This cell line is a suspension cell line. Harvest the cell suspension by centrifugation; do not discard the culture medium directly.
Complete Medium	IMDM [PM150510]+20% Nutrient+1% Supplement
Incubation Atmosphere	Air, 95%; CO₂, 5%
Temperature	37℃
Subcultivation Ratio	3×10⁵-5×10⁵ cells/mL
Medium Renewal	2 to 3 times per week
Subculturing Procedure	Cultures can be maintained by either supplementing with fresh medium or exchanging medium via centrifugation. The recommended centrifugation conditions are 1200 rpm (approximately 250 × g) for 3 minutes.
Freeze Medium	General Freezing Medium [PB180436]
Storage Conditions	For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150°C to −196°C. Storage at −80°C is restricted to short-term interim use only.

Reference materials

Background	The HuT 78 cell line was isolated and established in 1981 by A.F. Gazdar from the peripheral blood of a 53-year-old Caucasian male with Sézary syndrome. HuT 78 cells display the inducer/helper phenotype characteristic of mature T cells. Biologically active IL-2 can be eluted from the cell surface.
Tumorigenic	Yes, in nude mice when injected intracranially.
Receptor Expression	interleukin 2 (IL-2), expressed
Antigen Expression	CD4; Homo sapiens
Gene Expression	interleukin 2, tumor necrosis factor alpha (TNF alpha), CD4; Homo sapiens
Biosafety Level	BSL-1