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Product Introduction
| Cell Name | J82 |
| Synonyms | J-82;J 82;J82COT;J82 COT |
| Cellosaurus Accession | CVCL_0359 |
| Organism | Human |
| Growth Properties | Adherent |
| Morphology | Epithelial-like |
| Disease | bladder cancer cells |
| Tissue | Urinary bladder |
| Age | 58Y |
| Sex | Male |
| Instructions | 1. Check all containers for leakage or breakage. 2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation. |
| Complete Medium | MEM, with NEAA [PM150410]+10% Nutrient+1% Supplement |
| Incubation Atmosphere | Air, 95%; CO₂, 5% |
| Temperature | 37℃ |
| Subcultivation Ratio | 1:2-1:4 |
| Medium Renewal | 2 to 3 times per week |
| Dissociation Duration | 2-3 min |
| Subculturing Procedure | 1. Remove the culture medium from the T25 cell culture flask. 2. Add approximately 2 mL of PBS. Gently tilt the flask side to side until the PBS covers the entire bottom, then aspirate and discard the PBS. 3. Add 1 mL of 0.25% trypsin solution (containing EDTA). Gently tilt the flask side to side until the trypsin solution covers the entire bottom of the flask. 4. Incubate the cells at 37°C. Observe the cells under an inverted microscope and terminate digestion once the cells round up and detach. To avoid clumping, do not agitate the cells by tapping or shaking the flask during detachment. 5. Add 3 mL of complete culture medium to terminate digestion and disperse into a single cell suspension. 6. Collect the cell suspension and centrifuge at 1200 rpm (approximately 250 ×g) for 3 minutes. Carefully aspirate and discard the supernatant. 7. Add fresh complete culture medium, pipette gently several times to resuspend the cells, and seed them at the appropriate ratio into a new culture flask. Loosen the cap or use a vented cap for incubation. |
| Freeze Medium | Freezing Medium (Serum-free & animal origin-free) [PB180438] |
| Storage Conditions | For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150°C to −196°C. Storage at −80°C is restricted to short-term interim use only. |
| Background | J82 cells were derived from a 58-year-old Caucasian male with bladder transitional cell carcinoma. The cells lack desmosomes, possess abundant rough endoplasmic reticulum and prominent microvilli, and express the ras oncogene. |
| Tumorigenic | Yes, in nude mice. |
| Antigen Expression | HLA A2, Aw32, B5, B12, Cw5; Blood Type A |
| Biosafety Level | BSL-1 |
Documents
MSDSPublications
Journal: DRUG DEVELOPMENT RESEARCH (2024) IF: 3.5
DOI: 10.1002/ddr.22242
Product Cited: McCoy's 5A Medium, J82 Cells Line, Penicillin-Streptomycin Solution, 100 ×, MEM, with NEAA Medium, Fetal Bovine Serum, T24 [T-24] Cells Line
Journal: PATHOLOGY RESEARCH AND PRACTICE (2024) IF: 2.8
DOI: 10.1016/j.prp.2024.155369
Product Cited: T24 [T-24] Cells Line, SV-HUC-1 Cells Line, J82 Cells Line, UM-UC-3 Cells Line
Journal: MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS (2024) IF: 2.3
DOI: 10.1016/j.mrfmmm.2024.111876
Product Cited: Fetal Bovine Serum, 5637 Cells Line, RPMI-1640 Medium, McCoy's 5A Medium, T24 [T-24] Cells Line, Ham's F-12K Medium, J82 Cells Line, DMEM (High glucose) Medium, MEM, with NEAA Medium, 293T [HEK-293T] Cells Line, Penicillin-Streptomycin Solution, 100 ×, SV-HUC-1 Cells Line, RT4 Cells Line
Journal: Open Life Sciences (2024) IF: 1.7
Product Cited: T24 [T-24] Cells Line, J82 Cells Line, SV-HUC-1 Cells Line
Journal: CANCER LETTERS (2023) IF: 9.7
DOI: 10.1016/j.canlet.2023.216058
Product Cited: SV-HUC-1 Cells Line, UM-UC-3 Cells Line, J82 Cells Line, 5637 Cells Line, SW 780 [SW-780, SW780] Cells Line, T24 [T-24] Cells Line, TCCSUP Cells Line, Ham's F-12K, MEM(with NEAA), Leibovitz's L-15, McCoy's 5A
