JEG-3

Name: JEG-3
Brand: Procell
SKU: CL-0127
Price: 420.0 USD
Availability: InStock

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Cat. No.: CL-0127

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CL-0127

$ 420.00

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1×10^6Cells/Vial×2Vials

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Product Introduction Documents Publications

Product Introduction

General information

Cell Name	JEG-3
Synonyms	Jeg-3;JEG3;Jeg3;jeg3
Cellosaurus Accession	CVCL_0363
Organism	Human
Growth Properties	Adherent
Morphology	Epithelial-like
Disease	other cancer cells
Tissue	Placenta; derived from metastatic site: cerebral metastasis
Age	Fetus
Sex	Male

Handling information

Instructions	1. Check all containers for leakage or breakage. 2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation.
Complete Medium	MEM, with NEAA [PM150410]+10% Nutrient+1% Supplement
Incubation Atmosphere	Air, 95%; CO₂, 5%
Temperature	37℃
Subcultivation Ratio	1:3-1:6
Medium Renewal	2 to 3 times per week
Dissociation Duration	2-3 min
Subculturing Procedure	1. Remove the culture medium from the T25 cell culture flask. 2. Add approximately 2 mL of PBS. Gently tilt the flask side to side until the PBS covers the entire bottom, then aspirate and discard the PBS. 3. Add 1 mL of 0.25% trypsin solution (containing EDTA). Gently tilt the flask side to side until the trypsin solution covers the entire bottom of the flask. 4. Incubate the cells at 37°C. Observe the cells under an inverted microscope and terminate digestion once the cells round up and detach. To avoid clumping, do not agitate the cells by tapping or shaking the flask during detachment. 5. Add 3 mL of complete culture medium to terminate digestion and disperse into a single cell suspension. 6. Collect the cell suspension and centrifuge at 1200 rpm (approximately 250 ×g) for 3 minutes. Carefully aspirate and discard the supernatant. 7. Add fresh complete culture medium, pipette gently several times to resuspend the cells, and seed them at the appropriate ratio into a new culture flask. Loosen the cap or use a vented cap for incubation.
Freeze Medium	General Freezing Medium [PB180436]
Storage Conditions	For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150°C to −196°C. Storage at −80°C is restricted to short-term interim use only.

Reference materials

Background	JEG-3 cells are a hypertriploid human cell line. The modal chromosome number of JEG-3 cells is 71, occurring at 34%, with a polyploidy rate of 2.6%. The t(4;11)(p15;q13), i(13q), t(10p15q), del(18)(q21), and six other markers are common to most cells, while two additional markers are found in some cells. Giant satellites are observed on one N14 and two N22 chromosomes. Chromosomes N2, N5, and N9 are present in four copies, whereas N7, N13, N18, N21, and X are single copies. A single Y chromosome can be detected by Q-band examination.
Tumorigenic	Yes, in nude mice; forms malignant tumor consistent with choriocarcinoma.
Gene Expression	human chorionic gonadotropin (hCG), human chorionic somatomammotropin (placental lactogen); progesterone
Biosafety Level	BSL-1