Publications(
CL-0129
$ 420.00
Select a size
Quantity
In stock
InquireFor order requirements and technical needs, please Contact Us
Product Introduction
| Cell Name | Jurkat, Clone E6-1 |
| Synonyms | JurkatE6-1;Jurkat E6-1;Jurkat, Clone E6-1;Jurkat Clone E6-1;Jurkat (clone E6-1);JURKAT E-6.1;JURKAT E-61;Jurkat-E6;Jurkat E6;J.E6-1;E6-1 |
| Cellosaurus Accession | CVCL_0367 |
| Organism | Human |
| Growth Properties | Suspension |
| Morphology | Lymphoblast-like |
| Disease | leukemia cells |
| Tissue | Peripheral blood |
| Age | 14Y |
| Sex | Male |
| Instructions | 1. Check all containers for leakage or breakage. 2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation. 3. This cell line is a suspension cell line. Harvest the cell suspension by centrifugation; do not discard the culture medium directly. |
| Complete Medium | RPMI-1640 [PM150110]+10% Nutrient+1% Supplement |
| Incubation Atmosphere | Air, 95%; CO₂, 5% |
| Temperature | 37℃ |
| Subcultivation Ratio | 3×10⁵-5×10⁵ cells/mL |
| Medium Renewal | 2 to 3 times per week |
| Subculturing Procedure | Cultures can be maintained by either supplementing with fresh medium or exchanging medium via centrifugation. The recommended centrifugation conditions are 1200 rpm (approximately 250 × g) for 3 minutes. |
| Freeze Medium | General Freezing Medium [PB180436] |
| Storage Conditions | For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150°C to −196°C. Storage at −80°C is restricted to short-term interim use only. |
| Background | Jurkat, Clone E6-1 cells are a subclone of the Jurkat-FHCRC cell line, itself a derivative of the Jurkat cell line. The cells were derived from peripheral blood of a 14-year-old male patient and secrete high levels of IL-2 following stimulation with phorbol esters plus either lectins or anti-T3 monoclonal antibodies (both types of inducers are required). |
| Receptor Expression | T cell antigen receptor, expressed |
| Antigen Expression | CD3; Homo sapiens, expressed |
| Gene Expression | interleukin-2 (interleukin 2, IL-2), CD3; Homo sapiens, expressed |
| Biosafety Level | BSL-1 |
Documents
MSDSPublications
Journal: Cancer Immunology Research (2023) IF: 10.1
DOI: 10.1158/2326-6066.CIR-22-0439
Product Cited: Jurkat, Clone E6-1 Cells Line, RKO Cells Line, CT26.WT Cells Line, B16-F10 Cells Line, STR Identification, 4T1 Cells Line, LLC [LL/2; LLC1] Cells Line, Hepa 1-6 Cells Line, YAC-1 Cells Line, 293T [HEK-293T] Cells Line, AAV-293 Cells Line, STR Identification
Journal: PHARMACOLOGICAL RESEARCH (2023) IF: 9.3
DOI: 10.1016/j.phrs.2023.106686
Product Cited: Jurkat, Clone E6-1 Cells Line, MOLT-4 Cells Line, EL4 Cells Line
Journal: SENSORS AND ACTUATORS B-CHEMICAL (2023) IF: 8.4
DOI: 10.1016/j.snb.2023.134497
Product Cited: K-562 [K562] Cells Line, U-937 Cells Line, Raji Cells Line, Jurkat, Clone E6-1 Cells Line, K-562 [K562] Cells Complete Medium, U-937 Cells Complete Medium, Raji Cells Complete Medium, Jurkat, Clone E6-1 Cells Complete Medium
Journal: JOURNAL OF CELL BIOLOGY (2023) IF: 7.8
Product Cited: U-87 MG Cells Line, Jurkat, Clone E6-1 Cells Line, Raji Cells Line, A20 Cells Line
Journal: Journal of Translational Medicine (2023) IF: 7.4
DOI: 10.1186/s12967-023-04365-3
Product Cited: Jurkat, Clone E6-1 Cells Line
