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CL-0129
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Product Introduction
| Cell Name | Jurkat, Clone E6-1 |
| Synonyms | JurkatE6-1;Jurkat E6-1;Jurkat, Clone E6-1;Jurkat Clone E6-1;Jurkat (clone E6-1);JURKAT E-6.1;JURKAT E-61;Jurkat-E6;Jurkat E6;J.E6-1;E6-1 |
| Cellosaurus Accession | CVCL_0367 |
| Organism | Human |
| Growth Properties | Suspension |
| Morphology | Lymphoblast-like |
| Disease | leukemia cells |
| Tissue | Peripheral blood |
| Age | 14Y |
| Sex | Male |
| Instructions | 1. Check all containers for leakage or breakage. 2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation. 3. This cell line is a suspension cell line. Harvest the cell suspension by centrifugation; do not discard the culture medium directly. |
| Complete Medium | RPMI-1640 [PM150110]+10% Nutrient+1% Supplement |
| Incubation Atmosphere | Air, 95%; CO₂, 5% |
| Temperature | 37℃ |
| Subcultivation Ratio | 3×10⁵-5×10⁵ cells/mL |
| Medium Renewal | 2 to 3 times per week |
| Subculturing Procedure | Cultures can be maintained by either supplementing with fresh medium or exchanging medium via centrifugation. The recommended centrifugation conditions are 1200 rpm (approximately 250 × g) for 3 minutes. |
| Freeze Medium | General Freezing Medium [PB180436] |
| Storage Conditions | For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150°C to −196°C. Storage at −80°C is restricted to short-term interim use only. |
| Background | Jurkat, Clone E6-1 cells are a subclone of the Jurkat-FHCRC cell line, itself a derivative of the Jurkat cell line. The cells were derived from peripheral blood of a 14-year-old male patient and secrete high levels of IL-2 following stimulation with phorbol esters plus either lectins or anti-T3 monoclonal antibodies (both types of inducers are required). |
| Receptor Expression | T cell antigen receptor, expressed |
| Antigen Expression | CD3; Homo sapiens, expressed |
| Gene Expression | interleukin-2 (interleukin 2, IL-2), CD3; Homo sapiens, expressed |
| Biosafety Level | BSL-1 |
Documents
MSDSPublications
Journal: INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE (2026) IF: 5.8
Product Cited: PUMC-HUVEC-T1 Cells Line, THP-1 Cells Line, Jurkat, Clone E6-1 Cells Line, THP-1 Cell Complete Medium, Jurkat, Clone E6-1 Cell Complete Medium
Journal: Current Research in Microbial Sciences (2026) IF: 5.8
DOI: 10.1016/j.crmicr.2026.100572
Product Cited: Ramos Cells Line, C666-1 Cells Line, Jurkat, Clone E6-1 Cells Line, AGS Cells Line, Akata Cells Line, CNE-2Z Cells Line, B95-8 Cells Line, 293T Cells Line, HNE-1 Cells Line, Raji Cells Line, Daudi Cells Line
Journal: CANCER IMMUNOLOGY IMMUNOTHERAPY (2026) IF: 5.1
DOI: 10.1007/s00262-025-04283-x
Product Cited: SK-OV-3 Cells Line, Jurkat, Clone E6-1 Cells Line, Hey Cells Line
Journal: CANCER IMMUNOLOGY IMMUNOTHERAPY (2026) IF: 5.1
DOI: 10.1007/s00262-026-04353-8
Product Cited: Jurkat, Clone E6-1 Cells Line, THP-1 Cells Line, 253J Cells Line, SV-HUC-1 Cells Line, RT4 Cells Line, T24 Cells Line, TCCSUP Cells Line, MB49 Cells Line, J82 Cells Line, 5637 Cells Line, 293T Cells Line
Journal: MOLECULAR AND CELLULAR BIOCHEMISTRY (2026) IF: 3.7
DOI: 10.1007/s11010-025-05458-x
Product Cited: CCRF-CEM Cells Line, HuT 78 Cells Line, MOLT-4 Cells Line, Jurkat, Clone E6-1 Cells Line, H9 Cells Line, RPMI-1640 Medium
