Jurkat, Clone E6-1

Name: Jurkat, Clone E6-1
Brand: Procell
SKU: CL-0129
Price: 420.0 USD
Availability: InStock

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Cat. No.: CL-0129

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1×10^6Cells/Vial×2Vials

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Product Introduction Documents Publications

General information

Cell Name	Jurkat, Clone E6-1
Synonyms	JurkatE6-1;Jurkat E6-1;Jurkat, Clone E6-1;Jurkat Clone E6-1;Jurkat (clone E6-1);JURKAT E-6.1;JURKAT E-61;Jurkat-E6;Jurkat E6;J.E6-1;E6-1
Cellosaurus Accession	CVCL_0367
Organism	Human
Growth Properties	Suspension
Morphology	Lymphoblast-like
Disease	leukemia cells
Tissue	Peripheral blood
Age	14Y
Sex	Male

Handling information

Instructions	1. Check all containers for leakage or breakage. 2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation. 3. This cell line is a suspension cell line. Harvest the cell suspension by centrifugation; do not discard the culture medium directly.
Complete Medium	RPMI-1640 [PM150110]+10% Nutrient+1% Supplement
Incubation Atmosphere	Air, 95%; CO₂, 5%
Temperature	37℃
Subcultivation Ratio	3×10⁵-5×10⁵ cells/mL
Medium Renewal	2 to 3 times per week
Subculturing Procedure	Cultures can be maintained by either supplementing with fresh medium or exchanging medium via centrifugation. The recommended centrifugation conditions are 1200 rpm (approximately 250 × g) for 3 minutes.
Freeze Medium	General Freezing Medium [PB180436]
Storage Conditions	For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150°C to −196°C. Storage at −80°C is restricted to short-term interim use only.

Reference materials

Background	Jurkat, Clone E6-1 cells are a subclone of the Jurkat-FHCRC cell line, itself a derivative of the Jurkat cell line. The cells were derived from peripheral blood of a 14-year-old male patient and secrete high levels of IL-2 following stimulation with phorbol esters plus either lectins or anti-T3 monoclonal antibodies (both types of inducers are required).
Receptor Expression	T cell antigen receptor, expressed
Antigen Expression	CD3; Homo sapiens, expressed
Gene Expression	interleukin-2 (interleukin 2, IL-2), CD3; Homo sapiens, expressed
Biosafety Level	BSL-1