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CL-0129
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Product Introduction
| Cell Name | Jurkat, Clone E6-1 |
| Synonyms | JurkatE6-1;Jurkat E6-1;Jurkat, Clone E6-1;Jurkat Clone E6-1;Jurkat (clone E6-1);JURKAT E-6.1;JURKAT E-61;Jurkat-E6;Jurkat E6;J.E6-1;E6-1 |
| Cellosaurus Accession | CVCL_0367 |
| Organism | Human |
| Growth Properties | Suspension |
| Morphology | Lymphoblast-like |
| Disease | leukemia cells |
| Tissue | Peripheral blood |
| Age | 14Y |
| Sex | Male |
| Instructions | 1. Check all containers for leakage or breakage. 2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation. 3. This cell line is a suspension cell line. Harvest the cell suspension by centrifugation; do not discard the culture medium directly. |
| Complete Medium | RPMI-1640 [PM150110]+10% Nutrient+1% Supplement |
| Incubation Atmosphere | Air, 95%; CO₂, 5% |
| Temperature | 37℃ |
| Subcultivation Ratio | 3×10⁵-5×10⁵ cells/mL |
| Medium Renewal | 2 to 3 times per week |
| Subculturing Procedure | Cultures can be maintained by either supplementing with fresh medium or exchanging medium via centrifugation. The recommended centrifugation conditions are 1200 rpm (approximately 250 × g) for 3 minutes. |
| Freeze Medium | General Freezing Medium [PB180436] |
| Storage Conditions | For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150°C to −196°C. Storage at −80°C is restricted to short-term interim use only. |
| Background | Jurkat, Clone E6-1 cells are a subclone of the Jurkat-FHCRC cell line, itself a derivative of the Jurkat cell line. The cells were derived from peripheral blood of a 14-year-old male patient and secrete high levels of IL-2 following stimulation with phorbol esters plus either lectins or anti-T3 monoclonal antibodies (both types of inducers are required). |
| Receptor Expression | T cell antigen receptor, expressed |
| Antigen Expression | CD3; Homo sapiens, expressed |
| Gene Expression | interleukin-2 (interleukin 2, IL-2), CD3; Homo sapiens, expressed |
| Biosafety Level | BSL-1 |
Documents
MSDSPublications
Journal: Journal of Translational Medicine (2025) IF: 7.5
DOI: 10.1186/s12967-025-06866-9
Product Cited: HCC827 Cells Line, BEAS-2B Cells Line, Jurkat, Clone E6-1 Cells Line, A549 Cells Line, PC-9 Cells Line, 293T Cells Line, NCI-H1975 Cells Line, NCI-H1299 Cells Line, H1734 Cells Line
Journal: ANALYTICAL CHEMISTRY (2025) IF: 6.7
DOI: 10.1021/acs.analchem.5c04651
Product Cited: T3M4 Cells Line, NCI-H292 Cells Line, Jurkat, Clone E6-1 Cells Line, BxPC-3 Cells Line, SK-BR-3 Cells Line
Journal: ANALYTICAL CHEMISTRY (2025) IF: 6.7
DOI: 10.1021/acs.analchem.5c05244
Product Cited: A549 Cells Line, Hela Cells Line, MCF7 Cells Line, Jurkat, Clone E6-1 Cells Line
Journal: EMBO REPORTS (2025) IF: 6.5
DOI: 10.1038/s44319-025-00385-w
Product Cited: RPMI-1640 Medium, SK-BR-3 [SKBR3] Cells Line, T-47D Cells Line, HCC1937 Cells Line, BT-549 Cells Line, ZR-75-1 Cells Line, JeKo-1 Cells Line, 293FT Cells Line, MDA-MB-436 Cells Line, Jurkat, Clone E6-1 Cells Line, McCoy's 5A Medium, DMEM (High glucose) Medium, BT-474 [BT474] Cells Line
Journal: ImmunoTargets and Therapy (2025) IF: 6.2
DOI: 10.2147/ITT.S494696
Product Cited: Jurkat, Clone E6-1 Cells Line
