K-562-GFP
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Cat. No.: CL-1071
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CL-1071
$ 720.00
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1×10^6Cells/Vial×2Vials
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Product Introduction
General information
| Cell Name | K-562-GFP |
| Organism | Human |
| Growth Properties | Suspension |
| Morphology | Lymphoblast-like |
| Disease | leukemia cells |
| Tissue | Bone Marrow |
| Age | 53Y |
| Sex | Female |
Handling information
| Instructions | 1. Check all containers for leakage or breakage. 2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation. 3. This cell line is a suspension cell line. Harvest the cell suspension by centrifugation; do not discard the culture medium directly. |
| Complete Medium | IMDM [PM150510]+10% Nutrient+1% Supplement |
| Incubation Atmosphere | Air, 95%; CO₂, 5% |
| Temperature | 37℃ |
| Subcultivation Ratio | 3×10⁵-5×10⁵ cells/mL |
| Medium Renewal | 2 to 3 times per week |
| Subculturing Procedure | Cultures can be maintained by either supplementing with fresh medium or exchanging medium via centrifugation. The recommended centrifugation conditions are 1200 rpm (approximately 250 × g) for 3 minutes. |
| Freeze Medium | General Freezing Medium [PB180436] |
| Storage Conditions | For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150°C to −196°C. Storage at −80°C is restricted to short-term interim use only. |
Reference materials
| Background | The continuous cell line K-562 was established by Lozzio from the pleural effusion of a terminally ill 53-year-old female patient with chronic myelogenous leukemia. K-562 cells were originally classified as highly undifferentiated granulocytic cells; however, studies by Anderson et al. on their surface membrane properties identified K-562 as a human erythroleukemia cell line. K-562 cells are widely used as highly sensitive in vitro targets in natural killer (NK) cell assays. Pross et al. employed K-562 cells for the detailed in vitro detection of NK cells, including the mathematical quantification of NK cell activity. K-562 blasts are pluripotent hematopoietic malignant cells capable of spontaneous differentiation into recognizable erythroid progenitors, granulocytes, monocytes, and other lineages. K-562 cells are EBNA-negative. The K-562-GFP cell line was constructed from K-562 cells by lentiviral transduction and is a stable cell line capable of long-term, high-level GFP expression. This cell line exhibits low passage, high viability, and good condition, making it suitable for assays such as flow cytometry and fluorescence imaging. Selection drug concentration: Puro = 7.0 μg/mL. |
| Biosafety Level | BSL-2 |
MSDS
