K-562 [K562]

Name: K-562 [K562]
Brand: Procell
SKU: CL-0130
Price: 420.0 USD
Availability: InStock

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Cat. No.: CL-0130

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1×10^6Cells/Vial×2Vials

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General information

Handling information

Instructions	1. Check all containers for leakage or breakage. 2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation. 3. This cell line is a suspension cell line. Harvest the cell suspension by centrifugation; do not discard the culture medium directly.
Complete Medium	IMDM [PM150510]+10% Nutrient+1% Supplement
Incubation Atmosphere	Air, 95%; CO₂, 5%
Temperature	37℃
Subcultivation Ratio	3×10⁵-5×10⁵ cells/mL
Medium Renewal	2 to 3 times per week
Subculturing Procedure	Cultures can be maintained by either supplementing with fresh medium or exchanging medium via centrifugation. The recommended centrifugation conditions are 1200 rpm (approximately 250 × g) for 3 minutes.
Freeze Medium	General Freezing Medium [PB180436]
Storage Conditions	For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150°C to −196°C. Storage at −80°C is restricted to short-term interim use only.

Reference materials

Background	K-562 [K562] is a continuous cell line established by Lozzio from the pleural effusion of a terminally ill 53-year-old female patient with chronic myelogenous leukemia. Initially categorized as highly undifferentiated granulocytic cells, K-562 was later reclassified as a human erythroleukemia cell line following surface membrane property analyses conducted by Anderson et al. K-562 serves as a highly sensitive in vitro target widely utilized in natural killer (NK) cell functional assays. Pross et al. used K-562 to develop detailed in vitro detection methods for NK cells, including mathematical quantification of NK cell cytotoxic activity. K-562 blasts are pluripotent malignant hematopoietic cells that spontaneously differentiate into identifiable erythroid progenitors, granulocytes, monocytes, and other hematopoietic lineages. K-562 cells are EBNA-negative.
Tumorigenic	Yes, in nude mice (Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 1×10⁷ cells).
Biosafety Level	BSL-1