KHYG-1

Name: KHYG-1
Brand: Procell
SKU: CL-0903
Price: 420.0 USD
Availability: InStock

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Cat. No.: CL-0903

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1×10^6Cells/Vial×2Vials

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Product Introduction Documents Publications

General information

Cell Name	KHYG-1
Synonyms	KHYG1;KHYG
Cellosaurus Accession	CVCL_2976
Organism	Human
Growth Properties	Suspension
Morphology	Lymphoblast-like; round; single cells
Disease	leukemia cells
Tissue	Peripheral blood
Age	45Y
Sex	Female

Handling information

Instructions	1. Check all containers for leakage or breakage. 2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation. 3. This cell line is relatively difficult to recover. Some dead cells will be present after recovery, manifesting as dark granules in the background; the proliferation rate is slow. 4. An appropriate increase in IL-2 is recommended for weekend culture. 5. Minimize centrifugation during cell culture.
Complete Medium	RPMI-1640 [PM150110]+100 U/mL recombinant IL-2+10% Nutrient+1% Supplement
Incubation Atmosphere	Air, 95%; CO₂, 5%
Temperature	37℃
Subcultivation Ratio	5×10⁵-1 ×10⁶ cells/mL
Medium Renewal	2 to 3 times per week
Subculturing Procedure	Cultures can be maintained by either supplementing with fresh medium or exchanging medium via centrifugation. The recommended centrifugation conditions are 1200 rpm (approximately 250 × g) for 3 minutes.
Freeze Medium	90% FBS+10% DMSO
Storage Conditions	For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150°C to −196°C. Storage at −80°C is restricted to short-term interim use only.

Reference materials

Background	KHYG-1 was established 1997 from the peripheral blood of a 45-year-old woman with aggressive natural killer cell (NK) leukemia; cells were described in the literature to display strong cytotoxic activity and to carry a point mutation in exon 7 of the TP53 (p53) gene.
Antigen Expression	CD2 +, cyCD3 +, smCD3 -, CD4 -, CD5 -, CD6 +, CD7 +, CD8 +, CD13 -, CD19 -, CD34 -, TCRalpha/beta -, TCRgamma/delta -
Biosafety Level	BSL-1