KMS-11

Name: KMS-11
Brand: Procell
SKU: CL-0794
Price: 420.0 USD
Availability: InStock

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Cat. No.: CL-0794

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1×10^6Cells/Vial×2Vials

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Product Introduction Documents Publications

General information

Cell Name	KMS-11
Cellosaurus Accession	CVCL_2989
Organism	Human
Growth Properties	Semi-adherent and semi-suspension
Morphology	Lymphoblast-like
Disease	myeloma cells
Tissue	Pleural effusion
Age	67Y
Sex	Female

Handling information

Instructions	1. Check all containers for leakage or breakage. 2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation.
Complete Medium	RPMI-1640 [PM150110]+10% Nutrient+1% Supplement
Incubation Atmosphere	Air, 95%; CO₂, 5%
Temperature	37℃
Subcultivation Ratio	3×10⁵-5×10⁵ cells/mL
Medium Renewal	every 2 to 3 days
Dissociation Duration	7-10 min
Subculturing Procedure	1. This cell line displays mixed adherent and suspended phenotypes. Although suspended cells remain viable, their density is low and they may be aspirated and discarded directly. 2. Rinse the adherent cells with PBS, then add 1–2 mL of 0.25% Trypsin-EDTA to the culture flask. Incubate at 37°C. Once the cells round up and detach from the vessel surface, add 4–6 mL of complete medium to terminate digestion. Gently pipette to generate a single-cell suspension, then harvest cells by centrifugation. 3. Seed the cells into fresh culture vessels at the recommended split ratio.
Freeze Medium	Freezing Medium (Serum-free & animal origin-free) [PB180438]
Storage Conditions	For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150°C to −196°C. Storage at −80°C is restricted to short-term interim use only.