KYSE-140

Name: KYSE-140
Brand: Procell
SKU: CL-0953
Price: 420.0 USD
Availability: InStock

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Cat. No.: CL-0953

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CL-0953

$ 420.00

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1×10^6Cells/Vial×2Vials

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Product Introduction Documents Publications

Product Introduction

General information

Cell Name	KYSE-140
Synonyms	KYSE 140;Kyse-140;KYSE140;Kyse140
Cellosaurus Accession	CVCL_1347
Organism	Human
Growth Properties	Adherent
Morphology	Epithelial-like
Tissue	Esophagus
Age	54Y
Sex	Male

Handling information

Instructions	1. Check all containers for leakage or breakage. 2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation. 3. This cell line is refractory to dissociation. Wash twice with PBS. Soaking digestion is not recommended, as it will cause significant cell loss. Resuspend with culture medium only when individual single cells are observed under a microscope. This cell line requires a longer dissociation time. Wait until cell morphology becomes clearly rounded, then terminate dissociation with the corresponding culture medium. If dissociation is incomplete, many cells will remain attached to the culture dish; forcibly dislodging them will also result in abnormal cell morphology.
Complete Medium	RPMI-1640 [PM150110]+10% Nutrient+1% Supplement
Incubation Atmosphere	Air, 95%; CO₂, 5%
Temperature	37℃
Subcultivation Ratio	1:3-1:4
Medium Renewal	2 to 3 times per week
Dissociation Duration	5-8 min
Subculturing Procedure	1. Remove the culture medium from the T25 cell culture flask. 2. Add approximately 2 mL of PBS. Gently tilt the flask side to side until the PBS covers the entire bottom, then aspirate and discard the PBS. 3. Add 1 mL of 0.25% trypsin solution (containing EDTA). Gently tilt the flask side to side until the trypsin solution covers the entire bottom of the flask. 4. Incubate the cells at 37°C. Observe the cells under an inverted microscope and terminate digestion once the cells round up and detach. To avoid clumping, do not agitate the cells by tapping or shaking the flask during detachment. 5. Add 3 mL of complete culture medium to terminate digestion and disperse into a single cell suspension. 6. Collect the cell suspension and centrifuge at 1200 rpm (approximately 250 ×g) for 3 minutes. Carefully aspirate and discard the supernatant. 7. Add fresh complete culture medium, pipette gently several times to resuspend the cells, and seed them at the appropriate ratio into a new culture flask. Loosen the cap or use a vented cap for incubation.
Freeze Medium	Freezing Medium (Serum-free & animal origin-free) [PB180438]
Storage Conditions	For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150°C to −196°C. Storage at −80°C is restricted to short-term interim use only.

Reference materials

Background	The KYSE-140 cell line was derived from a moderately differentiated invasive squamous cell carcinoma resected from the middle intra-thoracic esophagus of a 54-year-old man prior to treatment (depth of invasion was a1). The cell line has been reported in the literature to carry a p53 mutation.
Biosafety Level	BSL-1