KYSE-510

Name: KYSE-510
Brand: Procell
SKU: CL-0737
Price: 500.0 USD
Availability: InStock

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Cat. No.: CL-0737

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CL-0737

$ 500.00

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1×10^6Cells/Vial×2Vials

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Product Introduction Documents Publications

Product Introduction

General information

Cell Name	KYSE-510
Synonyms	KYSE 510;Kyse-510;KYSE510;Kyse510
Cellosaurus Accession	CVCL_1354
Organism	Human
Growth Properties	Adherent
Morphology	Epithelial-like; grow as a monolayer with multilayered foci
Tissue	Esophagus
Age	67Y
Sex	Female

Handling information

Instructions	1. Check all containers for leakage or breakage. 2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation.
Complete Medium	44.5% RPMI-1640 [PM150110]+44.5% Ham's F-12 [PM150810]+10% Nutrient+1% Supplement
Incubation Atmosphere	Air, 95%; CO₂, 5%
Temperature	37℃
Subcultivation Ratio	1:3-1:5
Medium Renewal	every 2 to 3 days
Dissociation Duration	3-5 min
Subculturing Procedure	1. Remove the culture medium from the T25 cell culture flask. 2. Add approximately 2 mL of PBS. Gently tilt the flask side to side until the PBS covers the entire bottom, then aspirate and discard the PBS. 3. Add 1 mL of 0.25% trypsin solution (containing EDTA). Gently tilt the flask side to side until the trypsin solution covers the entire bottom of the flask. 4. Incubate the cells at 37°C. Observe the cells under an inverted microscope and terminate digestion once the cells round up and detach. To avoid clumping, do not agitate the cells by tapping or shaking the flask during detachment. 5. Add 3 mL of complete culture medium to terminate digestion and disperse into a single cell suspension. 6. Collect the cell suspension and centrifuge at 1200 rpm (approximately 250 ×g) for 3 minutes. Carefully aspirate and discard the supernatant. 7. Add fresh complete culture medium, pipette gently several times to resuspend the cells, and seed them at the appropriate ratio into a new culture flask. Loosen the cap or use a vented cap for incubation.
Freeze Medium	Freezing Medium (Serum-free & animal origin-free) [PB180438]
Storage Conditions	For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150°C to −196°C. Storage at −80°C is restricted to short-term interim use only.

Reference materials

Background	KYSE-510 is a human esophageal squamous cell carcinoma cell line derived from moderately adventitia-invasive, well-differentiated cervical esophageal tumor tissue of a 67-year-old female patient who received prior cisplatin-based chemotherapy and radiotherapy. Genetically, KYSE-510 harbors a TP53 mutation and exhibits amplification of MYC, HST1, and CCND1 (Cyclin D1). In vivo, this cell line is capable of forming well-differentiated tumors in nude mice.
Receptor Expression	unknown
Antigen Expression	unknown
Gene Expression	unknown
Biosafety Level	BSL-1