LAMA-84
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Cat. No.: CL-0961
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CL-0961
$ 420.00
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1×10^6Cells/Vial×2Vials
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Product Introduction
General information
| Cell Name | LAMA-84 |
| Synonyms | Lama-84;LAMA84;Lama84 |
| Cellosaurus Accession | CVCL_0388 |
| Organism | Human |
| Growth Properties | Suspension |
| Morphology | Lymphoblast-like; round; single cells |
| Disease | leukemia cells |
| Tissue | Peripheral blood |
| Age | 29Y |
| Sex | Female |
Handling information
| Instructions | 1. Check all containers for leakage or breakage. 2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation. 3. This cell line adheres to surfaces during culture and must be maintained in non-TC-treated culture flasks. |
| Complete Medium | RPMI-1640 [PM150110]+20% Nutrient+1% Supplement |
| Incubation Atmosphere | Air, 95%; CO₂, 5% |
| Temperature | 37℃ |
| Subcultivation Ratio | 1×10⁵-1×10⁶ cells/mL |
| Medium Renewal | 2 to 3 times per week |
| Subculturing Procedure | Cultures can be maintained by either supplementing with fresh medium or exchanging medium via centrifugation. The recommended centrifugation conditions are 1200 rpm (approximately 250 × g) for 3 minutes. |
| Freeze Medium | General Freezing Medium [PB180436] |
| Storage Conditions | For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150°C to −196°C. Storage at −80°C is restricted to short-term interim use only. |
Reference materials
| Background | LAMA-84 is a human cell line derived from the peripheral blood of a patient with chronic myeloid leukemia (CML) in blast crisis. This cell line is characterized by the presence of the Philadelphia chromosome, which gives rise to the BCR-ABL fusion gene, a hallmark of CML. The BCR-ABL oncogene is known for its role in elevating tyrosine kinase activity, which activates multiple signaling pathways leading to uncontrolled cell proliferation and resistance to apoptosis. Therefore, LAMA-84 cells are a valuable model for studying the molecular mechanisms of CML progression and for evaluating the efficacy of tyrosine kinase inhibitors (TKIs) in preclinical settings. In research, LAMA-84 has been extensively used to investigate the biology of CML, particularly with regard to drug resistance and disease evolution. Studies using this cell line have helped elucidate cellular responses to different generations of TKIs, such as imatinib, dasatinib, and nilotinib. Furthermore, LAMA-84 has contributed to the development of novel therapeutic strategies aimed at overcoming TKI resistance, including the evaluation of combination therapies that target other signaling pathways synergistically dysregulated by the BCR-ABL fusion protein. |
| Antigen Expression | GPIIb/IIIa+, GPIIIa+ |
| Biosafety Level | BSL-1 |
MSDS
