LNCaP clone FGC

Name: LNCaP clone FGC
Brand: Procell
SKU: CL-0143
Price: 500.0 USD
Availability: InStock

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Cat. No.: CL-0143

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CL-0143

$ 500.00

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1×10^6Cells/Vial×2Vials

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Product Introduction Documents Publications

Product Introduction

General information

Cell Name	LNCaP clone FGC
Synonyms	LNCaP-Clone-FGC;LNCaP.FGC;LNCaP-FGC;LNCaP FGC;LNCaP-ATCC
Cellosaurus Accession	CVCL_1379
Organism	Human
Growth Properties	Adherent
Morphology	Epithelial-like
Disease	prostate cancer cells
Tissue	Prostate; derived from metastatic site: left supraclavicular lymph node
Age	50Y
Sex	Male

Handling information

Instructions	1. Check all containers for leakage or breakage. 2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation. 3. This cell line does not form a uniform monolayer; instead, it forms colonies. 4. This cell line causes rapid acidification of the culture medium and grows slowly; therefore, cells should not be disturbed within 48 hours after subculture. 5. Standard TC-treated flasks or dishes do not support good cell adherence, resulting in relatively high culture difficulty. Corning CellBIND culture flasks (Cat. No.:3289) or culture dishes coated with Poly-L-Lysine solution (Cat. No.:PB180523) are required.
Complete Medium	RPMI-1640 [PM150110]+10% Nutrient+1% Supplement
Incubation Atmosphere	Air, 95%; CO₂, 5%
Temperature	37℃
Subcultivation Ratio	1:3-1:4
Medium Renewal	2 to 3 times per week
Dissociation Duration	2-3 min
Thawing Procedure	若收到细胞大片脱落，请按照如下处理方式处理：<br/>1. 将培养瓶内所有培养基转入无菌离心管，离心收集细胞（1200 rpm 3 min）去除旧培养基；<br/>2. 用PBS重悬细胞，将所有细胞收集到一个离心管中，再次离心（1200 rpm 3 min）去除PBS；<br/>3. 加入1 mL左右0.25%胰酶重悬细胞，混匀即可，不能吹打太多次，放入培养箱消化3分钟。<br/>4. 消化好后，用移液枪轻轻吹打细胞悬液，使细胞团分散，迅速加入3-5 mL含血清的培养基混匀以终止消化，离心（1200 rpm 3 min）去除胰酶；<br/>5. 加入5 mL左右的细胞相应的完全培养基混匀，按比例接入无菌培养瓶/皿中（首次传代推荐1:2）。
Subculturing Procedure	1. Remove the culture medium from the T25 cell culture flask. 2. Add approximately 2 mL of PBS. Gently tilt the flask side to side until the PBS covers the entire bottom, then aspirate and discard the PBS. 3. Add 1 mL of 0.25% trypsin solution (containing EDTA). Gently tilt the flask side to side until the trypsin solution covers the entire bottom of the flask. 4. Incubate the cells at 37°C. Observe the cells under an inverted microscope and terminate digestion once the cells round up and detach. To avoid clumping, do not agitate the cells by tapping or shaking the flask during detachment. 5. Add 3 mL of complete culture medium to terminate digestion and disperse into a single cell suspension. 6. Collect the cell suspension and centrifuge at 1200 rpm (approximately 250 ×g) for 3 minutes. Carefully aspirate and discard the supernatant. 7. Add fresh complete culture medium, pipette gently several times to resuspend the cells, and seed them at the appropriate ratio into a new culture flask. Loosen the cap or use a vented cap for incubation.
Freeze Medium	Freezing Medium (Serum-free & animal origin-free) [PB180438]
Storage Conditions	For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150°C to −196°C. Storage at −80°C is restricted to short-term interim use only.

Reference materials

Background	LNCaP clone FGC is a human prostate cancer cell line isolated via needle aspiration biopsy from the left supraclavicular lymph node of a 50‑year‑old Caucasian male with blood type B+, who suffered from metastatic prostate carcinoma. LNCaP clone FGC cells respond to 5‑alpha‑dihydrotestosterone; this androgen modulates cellular proliferation and stimulates acid phosphatase secretion. LNCaP clone FGC cells do not grow as a homogeneous monolayer but form cell clusters, which can be dissociated by repeated gentle pipetting during subculturing. The cells exhibit weak substrate adhesion, cannot reach full confluence, and rapidly acidify culture medium. LNCaP clone FGC proliferates extremely slowly, so cultures should remain undisturbed within the initial 48 hours post‑passage. Most LNCaP clone FGC cells detach from the flask surface and float in medium during flask transportation. Upon receiving the shipped flask, incubate it under standard monolayer culture conditions for 24–48 hours to facilitate cell reattachment, followed by medium replacement with fresh culture medium. If necessary, collect all cell suspension from the flask, centrifuge at 300 × g for 15 minutes, resuspend the cell pellet in 10 mL complete medium, and seed into a single culture flask.
Tumorigenic	Yes, in soft agar.Yes, the cells are tumorigenic in nude mice.
Receptor Expression	androgen receptor, positive;estrogen receptor, positive
Gene Expression	human prostatic acid phosphatase; prostate specific antigen
Biosafety Level	BSL-1