LoVo

Name: LoVo
Brand: Procell
SKU: CL-0144
Price: 420.0 USD
Availability: InStock

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Cat. No.: CL-0144

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CL-0144

$ 420.00

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1×10^6Cells/Vial×2Vials

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Product Introduction Documents Publications

Product Introduction

General information

Cell Name	LoVo
Synonyms	LOVO
Cellosaurus Accession	CVCL_0399
Organism	Human
Growth Properties	Adherent
Morphology	Epithelial-like
Disease	colorectal cancer cells
Tissue	Colon; derived from metastatic site: supraclavicular region
Age	56Y
Sex	Male

Handling information

Instructions	1. Check all containers for leakage or breakage. 2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation.
Complete Medium	Ham's F-12K [PM150910]+10% Nutrient+1% Supplement
Incubation Atmosphere	Air, 95%; CO₂, 5%
Temperature	37℃
Subcultivation Ratio	1:2-1:4
Medium Renewal	2 to 3 times per week
Dissociation Duration	4-5 min
Subculturing Procedure	1. Remove the culture medium from the T25 cell culture flask. 2. Add approximately 2 mL of PBS. Gently tilt the flask side to side until the PBS covers the entire bottom, then aspirate and discard the PBS. 3. Add 1 mL of 0.25% trypsin solution (containing EDTA). Gently tilt the flask side to side until the trypsin solution covers the entire bottom of the flask. 4. Incubate the cells at 37°C. Observe the cells under an inverted microscope and terminate digestion once the cells round up and detach. To avoid clumping, do not agitate the cells by tapping or shaking the flask during detachment. 5. Add 3 mL of complete culture medium to terminate digestion and disperse into a single cell suspension. 6. Collect the cell suspension and centrifuge at 1200 rpm (approximately 250 ×g) for 3 minutes. Carefully aspirate and discard the supernatant. 7. Add fresh complete culture medium, pipette gently several times to resuspend the cells, and seed them at the appropriate ratio into a new culture flask. Loosen the cap or use a vented cap for incubation.
Freeze Medium	Freezing Medium (Serum-free & animal origin-free) [PB180438]
Storage Conditions	For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150°C to −196°C. Storage at −80°C is restricted to short-term interim use only.

Reference materials

Background	The LoVo cell line was established in 1971 from a tumor nodule that had metastasized to the left supraclavicular region of a 56-year-old Caucasian male diagnosed with colon adenocarcinoma. Oncogene testing has shown that LoVo cells are positive for the expression of C-myc, K-ras, H-ras, N-ras, Myb, sis, and fos; the expression of the N-myc oncogene was not tested. LoVo cells are tumorigenic in nude mice: when 1 × 10⁷ cells were subcutaneously inoculated, tumors developed in all 5 nude mice within 21 days. Additionally, LoVo cells express the tumor-specific nuclear matrix proteins CC-3 and CC-4.
Tumorigenic	Yes, in nude mice (Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 1×10⁷ cells).
Antigen Expression	HLA A11, B15, B17, Cw1, Cw3; Blood Type B
Gene Expression	carcinoembryonic antigen (CEA) 908 ng/10⁶ cells/10 days. The cells are negative for expression of CSAp (CSAp-) and colon antigen 3.
Biosafety Level	BSL-1