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LP-1

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Cat. No.: CL-1020 Publications(2)
LP-1 - 1
  • LP-1LP-1 - 1
  • LP-1LP-1 - 2
  • LP-1LP-1 - 3
  • +2

CL-1020

$ 500.00

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1×10^6Cells/Vial×2Vials

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Product Introduction

General information
Cell Name LP-1
Synonyms LP1
Cellosaurus Accession CVCL_0012
Organism Human
Growth Properties Suspension
Morphology Lymphoblast-like
Tissue Peripheral blood
Age 56Y
Sex 女性
Instructions 1. Check all containers for leakage or breakage.
2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation.
3. Cell density should be controlled; cells form aggregates at high densities.
Complete Medium IMDM [PM150510]+10% Nutrient+1% Supplement
Incubation Atmosphere Air, 95%; CO₂, 5%
Temperature 37℃
Subcultivation Ratio 1:2-1:3
Medium Renewal 2 to 3 times per week
Subculturing Procedure Cultures can be maintained by either supplementing with fresh medium or exchanging medium via centrifugation. The recommended centrifugation conditions are 1200 rpm (approximately 250 × g) for 3 minutes.
Freeze Medium General Freezing Medium [PB180436]
Storage Conditions For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150°C to −196°C. Storage at −80°C is restricted to short-term interim use only.
Background LP-1 was established from the peripheral blood of a 56-year-old woman with multiple myeloma (IgG, EBV-negative) in leukemic transformation (refractory, terminal) in 1986.
Biosafety Level BSL-1

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