LS 174T [LS174T]

Name: LS 174T [LS174T]
Brand: Procell
SKU: CL-0145
Price: 420.0 USD
Availability: InStock

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Cat. No.: CL-0145

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CL-0145

$ 420.00

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1×10^6Cells/Vial×2Vials

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Product Introduction Documents Publications

Product Introduction

General information

Cell Name	LS 174T [LS174T]
Synonyms	Ls174T;LS174t;Ls-174-T;LS-174-T;LS 174 T;LS-174T;Ls-174T;LS 174T;LS-174;LS174
Cellosaurus Accession	CVCL_1384
Organism	Human
Growth Properties	Adherent
Morphology	Epithelial-like
Disease	colorectal cancer cells
Tissue	Colon
Age	58Y
Sex	Female

Handling information

Instructions	1. Check all containers for leakage or breakage. 2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation.
Complete Medium	MEM (ATCC modified) [PM150467]+10% Nutrient+1% Supplement
Incubation Atmosphere	Air, 95%; CO₂, 5%
Temperature	37℃
Subcultivation Ratio	1:2-1:4
Medium Renewal	2 to 3 times per week
Dissociation Duration	2-3 min
Subculturing Procedure	1. Remove the culture medium from the T25 cell culture flask. 2. Add approximately 2 mL of PBS. Gently tilt the flask side to side until the PBS covers the entire bottom, then aspirate and discard the PBS. 3. Add 1 mL of 0.25% trypsin solution (containing EDTA). Gently tilt the flask side to side until the trypsin solution covers the entire bottom of the flask. 4. Incubate the cells at 37°C. Observe the cells under an inverted microscope and terminate digestion once the cells round up and detach. To avoid clumping, do not agitate the cells by tapping or shaking the flask during detachment. 5. Add 3 mL of complete culture medium to terminate digestion and disperse into a single cell suspension. 6. Collect the cell suspension and centrifuge at 1200 rpm (approximately 250 ×g) for 3 minutes. Carefully aspirate and discard the supernatant. 7. Add fresh complete culture medium, pipette gently several times to resuspend the cells, and seed them at the appropriate ratio into a new culture flask. Loosen the cap or use a vented cap for incubation.
Freeze Medium	General Freezing Medium [PB180436]
Storage Conditions	For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150°C to −196°C. Storage at −80°C is restricted to short-term interim use only.

Reference materials

Background	LS 174T [LS174T] is a trypsinized variant of the LS 180 cell line. It is more easily subcultured than its parental line and, like LS 180, produces high levels of carcinoembryonic antigen (CEA). Electron microscopy has revealed abundant microfilaments and intracytoplasmic mucin vacuoles in LS 174T cells. The cells are positive for rectal antigen 3; they are negative for p53 antigen expression but positive for p53 mRNA expression and stain positively for keratin. LS 174T cells show positive expression of the c-myc, N-myc, H-ras, N-ras, Myb, and fos oncogenes; expression of K-ras and sis was not tested.
Tumorigenic	Yes, in nude mice (Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 1×10⁷ cells).
Antigen Expression	serologically defined colon cancer antigen 3; Homo sapiens, expressed HLA A2, B13, B50; Blood Type O
Gene Expression	carcinoembryonic antigen (CEA), interleukin 10 (IL-10), interleukin 6 (IL-6), mucinThe production of CEA in the ATCC seed stock was 1944 ng per 10⁶ cells in 10 days.
Biosafety Level	BSL-1