M-07e

Name: M-07e
Brand: Procell
SKU: CL-0686
Price: 460.0 USD
Availability: InStock

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Cat. No.: CL-0686

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1×10^6Cells/Vial×2Vials

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Product Introduction Documents Publications

General information

Cell Name	M-07e
Synonyms	M-07E;M-O7e;M07-e;M07e;Mo7e;MO7e;M07E;MO7E
Cellosaurus Accession	CVCL_2106
Organism	Human
Growth Properties	Semi-adherent and semi-suspension
Morphology	Single round cells in suspension, a few cells may be spindle-like and slightly adherent
Disease	leukemia cells
Tissue	Peripheral blood
Age	6M
Sex	Female

Handling information

Instructions	1. Check all containers for leakage or breakage. 2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation. 3. The culture supernatant should be collected.
Complete Medium	RPMI-1640 [PM150110]+10% Nutrient+8 ng/mL Supplement1+1% Supplement2
Incubation Atmosphere	Air, 95%; CO₂, 5%
Temperature	37℃
Subcultivation Ratio	5×10⁵-1×10⁶ cells/mL
Medium Renewal	2 to 3 times per week
Dissociation Duration	1-2 min
Subculturing Procedure	1. This cell line displays mixed adherent and suspended phenotypes. Suspended cells remain viable. Transfer the single-cell suspension into a centrifuge tube and pellet the cells via centrifugation at 1200 × g (approximately 250 × g). 2. Adherent cells can be dispersed by gentle pipetting to form a homogeneous single-cell suspension. 3. Mix all harvested suspended and adherent cell populations, then seed the cells into fresh culture vessels at the recommended split ratio.
Freeze Medium	90% FBS+10% DMSO
Storage Conditions	For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150°C to −196°C. Storage at −80°C is restricted to short-term interim use only.

Reference materials

Background	M-07e is a cell line established in 1987 from the peripheral blood of a 6-month-old girl diagnosed with acute megakaryoblastic leukemia (AML M7) at initial diagnosis and represents a subclone of the growth factor-independent M-07 parental cell line. M-07e cells display proliferative responses to GM-CSF, IFN-α, IFN-β, IFN-γ, IL-2, IL-3, IL-4, IL-6, IL-15, NGF, SCF, TNF-α, and TPO. Notably, M-07e cells may rapidly acquire cytokine independence within 3–4 weeks of culture, likely resulting from the outgrowth of growth factor-autonomous subpopulations. Exome and RNA sequencing datasets are accessible for M-07e.
Antigen Expression	CD3 -, CD13 +, CD14 -, CD19 -, CD33 +, HLA-DR -
Biosafety Level	BSL-1