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Product Introduction
| Cell Name | Malme-3M |
| Synonyms | MALME-3M;MALME 3M;Malme-3 M;MALME.3M;Malme3M;MALME3M;Malme-3 Monolayer |
| Cellosaurus Accession | CVCL_1438 |
| Organism | Human |
| Growth Properties | Semi-adherent and semi-suspension |
| Morphology | Mixed |
| Disease | melanoma cells |
| Tissue | Skin; derived from metastatic site: lung |
| Age | 43Y |
| Sex | Male |
| Instructions | 1. Check all containers for leakage or breakage. 2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation. |
| Complete Medium | IMDM [PM150510]+20% Nutrient+1% Supplement |
| Incubation Atmosphere | Air, 95%; CO₂, 5% |
| Temperature | 37℃ |
| Subcultivation Ratio | 1:2 |
| Medium Renewal | 2 to 3 times per week |
| Dissociation Duration | 2-3 min |
| Subculturing Procedure | 1. This cell line displays mixed adherent and suspended phenotypes. Although suspended cells remain viable, their density is low and they may be aspirated and discarded directly. 2. Rinse the adherent cells with PBS, then add 1–2 mL of 0.25% Trypsin-EDTA to the culture flask. Incubate at 37°C. Once the cells round up and detach from the vessel surface, add 4–6 mL of complete medium to terminate digestion. Gently pipette to generate a single-cell suspension, then harvest cells by centrifugation. 3. Seed the cells into fresh culture vessels at the recommended split ratio. |
| Freeze Medium | General Freezing Medium [PB180436] |
| Storage Conditions | For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150°C to −196°C. Storage at −80°C is restricted to short-term interim use only. |
| Background | Malme-3M are fibroblasts that were isolated from the skin of a 43-year-old, White male patient with malignant melanoma. |
| Tumorigenic | Yes; Yes, in nude mice; forms pigmented malignant melanoma. |
| Antigen Expression | HLA A2, Aw30, B13, B40(+/-), DRw7 |
| Biosafety Level | BSL-1 |
Documents
MSDSPublications
Journal: Frontiers in Oncology (2024) IF: 3.5
DOI: 10.3389/fonc.2024.1471267
Product Cited: Hs 895.T Cells Line, WM793 Cells Line, HS-27A Cells Line, Hs 919.Sk Cells Line, A2058 Cells Line, SK-MEL-2 Cells Line, SK-MEL-28 Cells Line, Hs 895.B Cells Line, RPMI-7951 Cells Line, Hs 936.Sk Cells Line, Hs 895.D Cells Line, UACC-62 Cells Line, C32 Cells Line, Hs 852.T Cells Line, CCD-1140Sk Cells Line, CCD-1121Sk Cells Line, YUGEN8 Cells Line, CCD-1112Sk Cells Line, Hs 895.O Cells Line, Hs 888.Sk Cells Line, IPC-298 Cells Line, A-375 Cells Line, Hs 895.Sk Cells Line, G361 Cells Line, Hs 895.C Cells Line, Hs 895.A cell Cells Line, CCD-1106 KERTr Cells Line, LOX IMVI Cells Line, SK-MEL-5 Cells Line, WM35 Cells Line, MeWo Cells Line, Hs 895.R Cells Line, SK-MEL-3 Cells Line, Hs 895.P Cells Line, Malme-3M Cells Line, WM1552C Cells Line, CCD-1152Sk Cells Line, HMCB Cells Line, CCD-8Sk Cells Line, UACC-257 Cells Line, HS294T Cells Line
Journal: SENSORS AND ACTUATORS B-CHEMICAL (2023) IF: 8.4
DOI: 10.1016/j.snb.2023.135097
Product Cited: 4T1 Cells Line, B16 Cells Line, A549 [A-549] Cells Line, NCI-H1299 Cells Line, HL-7702 [L-02; LO2] Cells Line, BEAS-2B Cells Line, Hep G2 Cells Line, Malme-3M Cells Line
