MC/CAR

Name: MC/CAR
Brand: Procell
SKU: CL-0516
Price: 500.0 USD
Availability: InStock

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Cat. No.: CL-0516

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1×10^6Cells/Vial×2Vials

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Handling information

Instructions	1. Check all containers for leakage or breakage. 2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation.
Complete Medium	IMDM [PM150510]+20% Nutrient+1% Supplement
Incubation Atmosphere	Air, 95%; CO₂, 5%
Temperature	37℃
Subcultivation Ratio	1:2-1:4
Medium Renewal	2 to 3 times per week
Subculturing Procedure	Cultures can be maintained by either supplementing with fresh medium or exchanging medium via centrifugation. The recommended centrifugation conditions are 1200 rpm (approximately 250 × g) for 3 minutes.
Freeze Medium	General Freezing Medium [PB180436]
Storage Conditions	For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150°C to −196°C. Storage at −80°C is restricted to short-term interim use only.

Reference materials

Background	The MC/CAR cell line is a lymphoblast-like cell line isolated from the peripheral blood of an 81-year-old White male with plasmacytoma. This cell line can be used for immune system disorder research and as a human fusion partner cell line.
Tumorigenic	Yes; Yes, in nude mice inoculated subcutaneously with 10⁷ cells. (Tumors developed within 21 days at 100% frequency (5/5).)
Antigen Expression	HLA A1, A33/31, B7, B18, DRw1, DRw2; CD11a +; CD19 +; CD20 + CD28 -; CD38 +
Gene Expression	immunoglobulin
Biosafety Level	BSL-2