McCoy's 5A, without L-glutamine

Name: McCoy's 5A, without L-glutamine
Brand: Procell
SKU: PM150712
Price: 20.0 USD
Availability: InStock

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Cat. No.: PM150712

Manual

Formulation

PM150712

$ 20.00

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500mL

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Product Introduction Related Products Documents FAQs

Product Introduction

Background

McCoy's 5A medium was initially used specifically for the culture of Novikoff Hepatoma cells. Unlike other medium, McCoy's 5A medium contains reduced glutathione, bacterial peptone and high concentrations of glucose. McCoy's 5A is widely used in the culture of primary cells, such as bone marrow, skin, gingiva, kidney, spleen, lung, rat embryo, omentum and so on. In addition, McCoy's 5A medium is also used for tissue biopsy culture, the establishment of cell line, and the culture of some lymphocytes and more difficult cells.
This product contains many kinds of amino acids, vitamins, inorganic salts and other ingredients for cell culture, but does not contain protein, lipids or any growth factors. Therefore, the product should be used with serum or serum-free additives.

Ingredient Statement

With

• 3000 mg/L D-Glucose

• 2200 mg/L NaHCO₃

• 10 mg/L Phenol red

Without

• HEPES

• L-Glutamine

Matters Need Attention

1. This product is only used for scientific research or further research, not for diagnosis and treatment.
2. This product is sterilized by 0.1 μm filtration.
3. It is necessary to pay attention to the aseptic operation and avoid the contamination.

System Certification

GMP Production and Quality System
The company complies with cGMP requirements, holds the NMPA medical device registration certificate, and is certified under ISO 13485 and ISO 9001 standards. Among its products, the company's McCoy's 5A, without L-glutamine complies with ISO 13485, ISO 9001.

Specifications

Concentration	1×
Volume	500mL
Form	Liquid
Bacteria Detection	Negative
Fungi Detection	Negative
Mycoplasma Detection	Negative
Endotoxin Content	< 3 EU/mL
pH	7.2-7.4
Animal Origin Ingredient	Without
Green Features	Sustainable packaging
Valid Period	24 months
Storage Condition	This product can be stored at 2-8℃ for 24 months with shading light.
Shipping Condition	Room Temperature

Related Products

McCoy's 5A

Catalog Number: PM150710

McCoy's 5A, with HEPES, without L-glutamine

Catalog Number: PM150713

Documents

Manual

MSDS

Formulation

FAQs

Q:1 If the basic culture medium is frozen at -20℃, will it affect its use?
Answer
If the cell culture medium is accidentally frozen, observe whether there is precipitation after the medium is naturally thawed. If no precipitation is produced, the medium can be used normally. If precipitation occurs, it is recommended to discard it.
Q:2 How long can I keep my culture media after supplementing with serum?
Answer
Generally speaking, media can be used for up to 3-6 months after supplementation with serum， at 2 to 8℃ low temperature environment preservation.
Q:3 My medium was shipped at room temperature but it is supposed to be stored refrigerated. Is it okay?
Answer
We routinely ship media that require long-term storage in the refrigerator at room temperature. We have done studies on representative media formulations to show that media can be at room temperature for up to a week without a problem.
Q:4 How light sensitive is media? Should I also be protecting it from LED light?
Answer
While we know that different wavelengths of light are worse than others for exposure, we would recommend as a best practice to protect the medium from all forms of light exposure including LEDs, as much as possible to ensure optimal performance, as several components within the medium are light sensitive, such as vitamins.When placed in a light-proof 4°C refrigerator with the door closed, the culture medium will be kept away from light.
Q:5 I see a precipitate in my medium, and a change in the pH. What should I do?
Answer
This is most likely a sign of bacterial or fungal contamination. We would recommend discarding the media, and trying to decontaminate the culture.
Q:6 Where can I find the osmolality for Medium?
Answer
The osmolality is listed in the COA for the particular lot number of the medium.
Q:7 Do you provide customized culture media services?
Answer
Yes. We can customize culture media with reduced components or changes in the concentration of existing components.