MDA-MB-134-VI
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Cat. No.: CL-0906
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CL-0906
$ 500.00
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1×10^6Cells/Vial×2Vials
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Product Introduction
General information
| Cell Name | MDA-MB-134-VI |
| Synonyms | MDA-MB-134 VI;MDA MB 134VI;MDA-MB-134VI;MDAMB134VI;MDA-MB-134;MDAMB134;MDA-134;MDA134;MM134;MD Anderson-Metastatic Breast-134-VI |
| Cellosaurus Accession | CVCL_0617 |
| Organism | Human |
| Growth Properties | Semi-adherent and semi-suspension |
| Morphology | Epithelial-like |
| Disease | Invasive breast lobular carcinoma |
| Tissue | Breast; derived from metastatic site: pleural effusion |
| Age | 47Y |
| Sex | Female |
Handling information
| Instructions | 1. Check all containers for leakage or breakage. 2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation. 3. Leibovitz's L-15 medium is recommended for this cell line. CO₂ supplementation is not recommended for Leibovitz's L-15 medium, as this may cause cytotoxicity. 4. If a CO₂-free incubator is not available, DMEM may be used as an alternative to Leibovitz's L-15. When using DMEM medium, 5% CO₂ can be supplied normally. |
| Complete Medium | Leibovitz's L-15 [PM151010]+10% Nutrient+1% Supplement |
| Incubation Atmosphere | Air, 100% |
| Temperature | 37℃ |
| Subcultivation Ratio | 1:2-1:3 |
| Medium Renewal | 2 to 3 times per week |
| Dissociation Duration | 2-3 min |
| Subculturing Procedure | 1. This cell line displays mixed adherent and suspended phenotypes. Suspended cells remain viable. Transfer the single-cell suspension into a centrifuge tube and pellet the cells via centrifugation at 1200 × g (approximately 250 × g). 2. Weakly adherent cells can be dispersed by gentle pipetting to form a homogeneous single-cell suspension. 3. For firmly adherent cells, rinse with PBS, then add 1–2 mL of 0.25% Trypsin-EDTA to the culture flask. Incubate at 37°C. Once the cells round up and detach from the vessel surface, add 4–6 mL of complete medium to terminate digestion. Gently pipette to generate a single-cell suspension, then harvest cells by centrifugation. 4. Mix all harvested suspended and adherent cell populations, then seed the cells into fresh culture vessels at the recommended split ratio. |
| Freeze Medium | 90% FBS+10% DMSO |
| Storage Conditions | For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150°C to −196°C. Storage at −80°C is restricted to short-term interim use only. |
Reference materials
| Background | MDA-MB-134-VI is an epithelial-like cell that was isolated from the pleural effusion of a 47-year-old, White, female with ductal carcinoma. |
| Antigen Expression | Blood Type O; Rh+ |
| Biosafety Level | BSL-1 |
MSDS
