ME-180

Name: ME-180
Brand: Procell
SKU: CL-0155
Price: 420.0 USD
Availability: InStock

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Cat. No.: CL-0155

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CL-0155

$ 420.00

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1×10^6Cells/Vial×2Vials

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Product Introduction Documents Publications

Product Introduction

General information

Cell Name	ME-180
Synonyms	Me-180;ME 180;ME180
Cellosaurus Accession	CVCL_1401
Organism	Human
Growth Properties	Adherent
Morphology	Epithelial-like
Disease	cervical cancer cells
Tissue	Uterus; Cervix; derived from metastatic site: omentum
Age	66Y
Sex	Female

Handling information

Instructions	1. Check all containers for leakage or breakage.<br/> 2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation.
Complete Medium	McCoy's 5A [PM150710]+10% Nutrient+1% Supplement
Incubation Atmosphere	Air, 95%; CO₂, 5%
Temperature	37℃
Subcultivation Ratio	1:3-1:8
Medium Renewal	2 to 3 times per week
Dissociation Duration	2-3 min
Subculturing Procedure	1. Remove the culture medium from the T25 cell culture flask. 2. Add approximately 2 mL of PBS. Gently tilt the flask side to side until the PBS covers the entire bottom, then aspirate and discard the PBS. 3. Add 1 mL of 0.25% trypsin solution (containing EDTA). Gently tilt the flask side to side until the trypsin solution covers the entire bottom of the flask. 4. Incubate the cells at 37°C. Observe the cells under an inverted microscope and terminate digestion once the cells round up and detach. To avoid clumping, do not agitate the cells by tapping or shaking the flask during detachment. 5. Add 3 mL of complete culture medium to terminate digestion and disperse into a single cell suspension. 6. Collect the cell suspension and centrifuge at 1200 rpm (approximately 250 ×g) for 3 minutes. Carefully aspirate and discard the supernatant. 7. Add fresh complete culture medium, pipette gently several times to resuspend the cells, and seed them at the appropriate ratio into a new culture flask. Loosen the cap or use a vented cap for incubation.
Freeze Medium	Freezing Medium (Serum-free & animal origin-free) [PB180438]
Storage Conditions	For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150°C to −196°C. Storage at −80°C is restricted to short-term interim use only.

Reference materials

Background	The ME-180 cell line was established from invasive squamous cell carcinoma, which presents irregular cell aggregates with negligible keratinization. Zonulae adherentes can be observed between adjacent ME-180 cells in monolayer culture, along with cytoplasmic tonofilaments. This cell line was found to be mycoplasma-contaminated in 1970, and the contamination was later eliminated. Tumor necrosis factor-alpha (TNF-α) can suppress the proliferation of ME-180 cells. Furthermore, ME-180 cells harbor human papillomavirus (HPV) DNA, which shares higher sequence homology with HPV-39 than HPV-18.
Tumorigenic	Yes, in nude mice; forms well differentiated epidermoid carcinoma (grade I).
Antigen Expression	Blood Type A; Rh+; HLA A1, A11, B5(+/-), B40
Gene Expression	Oncogenes: p53+; pRB+
Biosafety Level	BSL-2