MEC-1
Copy the product info.
Cat. No.: CL-0761
-
-
-
- +2
CL-0761
$ 500.00
Select a size
1×10^6Cells/Vial×2Vials
Quantity
-
+
In stock
InquireFor order requirements and technical needs, please Contact Us
Product Introduction
General information
| Cell Name | MEC-1 |
| Synonyms | MEC1 |
| Cellosaurus Accession | CVCL_1870 |
| Organism | Human |
| Growth Properties | Suspension |
| Morphology | Round to pleomorphic cells growing in suspension, single or in small aggregates; a few cells weakly adherent |
| Disease | leukemia cells |
| Tissue | Peripheral blood |
| Age | 61Y |
| Sex | Male |
Handling information
| Instructions | 1. Check all containers for leakage or breakage. 2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation. |
| Complete Medium | IMDM [PM150510]+10% Nutrient+1% Supplement |
| Incubation Atmosphere | Air, 95%; CO₂, 5% |
| Temperature | 37℃ |
| Subcultivation Ratio | 1:2-1:3 |
| Medium Renewal | every 2 to 3 days |
| Subculturing Procedure | Cultures can be maintained by either supplementing with fresh medium or exchanging medium via centrifugation. The recommended centrifugation conditions are 1200 rpm (approximately 250 × g) for 3 minutes. |
| Freeze Medium | 90% FBS+10% DMSO |
| Storage Conditions | For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150°C to −196°C. Storage at −80°C is restricted to short-term interim use only. |
Reference materials
| Background | MEC-1 was established in 1993 from the peripheral blood of a 61-year-old male patient diagnosed with chronic B-cell leukemia exhibiting prolymphocytoid transformation to B-PLL. It is the serial sister cell line of MEC-2 (DSMZ ACC 500). Exome and RNA sequencing data are available (see Ref 18187 and Exome sequence and RNA-Seq datasets). |
| Biosafety Level | BSL-1 |
MSDS
