Mino

Name: Mino
Brand: Procell
SKU: CL-0779
Price: 500.0 USD
Availability: InStock

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Cat. No.: CL-0779

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1×10^6Cells/Vial×2Vials

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General information

Handling information

Instructions	1. Check all containers for leakage or breakage. 2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation.
Complete Medium	RPMI-1640 [PM150110]+20% Nutrient+1% Supplement
Incubation Atmosphere	Air, 95%; CO₂, 5%
Temperature	37℃
Subcultivation Ratio	1:2-1:3
Medium Renewal	2 to 3 times per week
Subculturing Procedure	Cultures can be maintained by either supplementing with fresh medium or exchanging medium via centrifugation. The recommended centrifugation conditions are 1200 rpm (approximately 250 × g) for 3 minutes.
Freeze Medium	General Freezing Medium [PB180436]
Storage Conditions	For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150°C to −196°C. Storage at −80°C is restricted to short-term interim use only.

Reference materials

Background	The Mino cell line consists of lymphoblasts isolated from the peripheral blood of a 64-year-old White male with mantle cell lymphoma (MCL). This cell line can be used for immunology research. The cells are large, growing singly and in small clumps in vitro. The immunophenotype determined by flow cytometry is compatible with MCL. Western blotting shows expression of cyclin D1 but no detectable cyclin D2 or cyclin D3; the retinoblastoma protein is predominantly phosphorylated. The cells express tumor suppressor gene products including p53, p16 (INK4a), and p21 (WAF1).
Antigen Expression	CD3-, CD5+, CD10-, CD19+, CD20+, CD23- (verified at ATCC)
Gene Expression	cyclin D2; cyclin D3; p53; p16 (INK4a); p21(WAF1)
Biosafety Level	BSL-1