Mitochondrial Membrane Potential Assay Kit (with JC-1)

Name: Mitochondrial Membrane Potential Assay Kit (with JC-1)
Brand: Procell
SKU: P-CA-301
Price: 108.0 USD
Availability: InStock

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Cat. No.: P-CA-301

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Mitochondrial Membrane Potential Assay Kit (with JC-1)Mitochondrial Membrane Potential Assay Kit (with JC-1) - 1

P-CA-301

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Product Introduction Documents Publications

Product Introduction

Detection Principle	The decrease of mitochondrial membrane potential is a marker event in the early stage of apoptosis. It occurs before the appearance of nuclear apoptotic features (chromatin condensation and DNA fragmentation). Once the mitochondrial membrane potential collapses, apoptosis is irreversible. JC-1 is an ideal fluorescent probe widely used to detect mitochondrial membrane potential ΔΨm. In normal cells, the mitochondrial membrane potential is high, JC-1 aggregates in the mitochondrial matrix to form a polymer, which can produce red fluorescence. In the early stage of apoptosis, the mitochondrial membrane potential decreases, JC-1 can’t accumulate in the mitochondrial matrix. When JC-1 is a monomer, it can produce green fluorescence. The decrease of cell membrane potential can be detected by the transition of JC-1 from red fluorescence to green fluorescence, and the transition of JC-1 fluorescence color can be used as an early detection indicator of cell apoptosis. The relative ratio of red and green fluorescence is commonly used to measure the ratio of mitochondrial depolarization. The maximum excitation wavelength of JC-1 monomer is 514 nm and the maximum emission wavelength is 529 nm; the maximum excitation wavelength of JC-1 polymer is 585 nm and the maximum emission wavelength is 590 nm.
Detection Method	Fluorometric method;Flow cytometry
Sample Type	Cell samples
Assay Time	40 min
Detection Instrument	Flow Cytometer;Fluorescence Microscope
Dye Type	JC-1
Ex/Em	514/529, 585/590
Channel Set	FITC, PE
Other Reagents Required	Ultrapure water
Storage	This product can be stored at 2-8°C/-5~-20°C for 12 months with shading light. Please refer to the manual for the specific storage condition of the components.
Expiration Date	12 months
Shipping	Ice bag