MM.1S

Name: MM.1S
Brand: Procell
SKU: CL-0614
Price: 420.0 USD
Availability: InStock

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Cat. No.: CL-0614

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1×10^6Cells/Vial×2Vials

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Product Introduction Documents Publications

General information

Cell Name	MM.1S
Synonyms	MM.1S;MM1-S;MM-1S;MM1S
Cellosaurus Accession	CVCL_8792
Organism	Human
Growth Properties	Semi-adherent and semi-suspension
Morphology	Round
Disease	myeloma cells
Tissue	Peripheral blood
Age	45Y
Sex	Female

Handling information

Instructions	1. Check all containers for leakage or breakage. 2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation.
Complete Medium	RPMI-1640 [PM150110]+10% Nutrient+1% Supplement
Incubation Atmosphere	Air, 95%; CO₂, 5%
Temperature	37℃
Subcultivation Ratio	1:2-1:4
Medium Renewal	2 to 3 times per week
Dissociation Duration	1-2 min
Subculturing Procedure	1. This cell line displays mixed adherent and suspended phenotypes. Suspended cells remain viable. Transfer the single-cell suspension into a centrifuge tube and pellet the cells via centrifugation at 1200 × g (approximately 250 × g). 2. Weakly adherent cells can be dispersed by gentle pipetting to form a homogeneous single-cell suspension. 3. For firmly adherent cells, rinse with PBS, then add 1–2 mL of 0.25% Trypsin-EDTA to the culture flask. Incubate at 37°C. Once the cells round up and detach from the vessel surface, add 4–6 mL of complete medium to terminate digestion. Gently pipette to generate a single-cell suspension, then harvest cells by centrifugation. 4. Mix all harvested suspended and adherent cell populations, then seed the cells into fresh culture vessels at the recommended split ratio.
Freeze Medium	90% FBS+10% DMSO
Storage Conditions	For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150°C to −196°C. Storage at −80°C is restricted to short-term interim use only.

Reference materials

Background	The parent cell line, MM.1, was established from peripheral blood of a multiple myeloma patient who had become resistant to steroid-based therapy. The closely related cell line, MM.1R was also isolated from MM.1, but is resistant to dexamethasone.
Receptor Expression	glucocorticoid receptor, expressed
Antigen Expression	CD25+, CD38+, CD52+, CD59+
Gene Expression	lambda-light chain immunoglobulin
Biosafety Level	BSL-1