MOLT-4

Name: MOLT-4
Brand: Procell
SKU: CL-0160
Price: 420.0 USD
Availability: InStock

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Cat. No.: CL-0160

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1×10^6Cells/Vial×2Vials

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Product Introduction Documents Publications

General information

Cell Name	MOLT-4
Synonyms	Molt-4;MOLT 4;Molt 4;MOLT.4;MOLT4;Molt4;GM02219;GM02219C;GM2219C
Cellosaurus Accession	CVCL_0013
Organism	Human
Growth Properties	Suspension
Morphology	Lymphoblast-like
Disease	leukemia cells
Tissue	Peripheral blood
Age	19Y
Sex	Male

Handling information

Instructions	1. Check all containers for leakage or breakage. 2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation. 3. This cell line is a suspension cell line. Harvest the cell suspension by centrifugation; do not discard the culture medium directly.
Complete Medium	RPMI-1640 [PM150110]+10% Nutrient+1% Supplement
Incubation Atmosphere	Air, 95%; CO₂, 5%
Temperature	37℃
Subcultivation Ratio	4×10⁵ cells/mL
Medium Renewal	2 to 3 times per week
Subculturing Procedure	Cultures can be maintained by either supplementing with fresh medium or exchanging medium via centrifugation. The recommended centrifugation conditions are 1200 rpm (approximately 250 × g) for 3 minutes.
Freeze Medium	General Freezing Medium [PB180436]
Storage Conditions	For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150°C to −196°C. Storage at −80°C is restricted to short-term interim use only.

Reference materials

Background	The MOLT-4 cell line was established from a patient with relapsed leukemia who had received prior multidrug chemotherapy. A G→A transition occurs at codon 248 of the TP53 gene, with no detectable p53 protein expression. MOLT-4 cells do not produce immunoglobulins or Epstein-Barr virus (EBV). MOLT-4 and MOLT-3 cells are derived from the same patient.
Tumorigenic	Yes, in untreated nude mice, anti lymphocyte serum treated mice and X-irradiated mice.
Antigen Expression	CD1 (49%), CD2 (35%), CD3 A (26%) B (33%) C (34%), CD4 (55%), CD5 (72%), CD6 (22%), CD7 (77%)
Gene Expression	high levels of terminal deoxynucleotidyl transferase (TdT) are produced.
Biosafety Level	BSL-1