Mouse Microglial Cells

Name: Mouse Microglial Cells
Brand: Procell
SKU: CP-M110
Price: 720.0 USD
Availability: InStock

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Cat. No.: CP-M110

Publications(11)

Manual

CP-M110

$ 720.00

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5×10^5Cells/Vial

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Product Introduction Documents Publications FAQs

Product Introduction

General Information

Cell Name	Mouse Microglial Cells
Species	Mouse
Tissue	Cerebral cortex tissue
Growth Properties	Adherent
Morphology	Spindle-shaped; polygonal
Isolation Method	Mouse microglial cells isolated from Procell Laboratory are prepared by enzymatic digestion, followed by differential adhesion. After several days of nutrient deprivation in culture medium, suspended cells are harvested via shaking incubation. Each vial contains approximately 5 × 10⁵ cells.
Identity Markers	Mouse microglial cells isolated from Procell Laboratory exhibit positive immunofluorescence staining for CD11b, with a purity greater than 90%. In addition, the cells test negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi.
Background	Mouse microglial cells (Mus musculus) are isolated from cerebral cortex tissue. They are the most predominant innate immune cells in the central nervous system, accounting for approximately 20% of the total glial cell population. Morphologically, they are equivalent to macrophages in the brain and spinal cord, and express ionized calcium-binding adapter molecule 1 (Iba1). Their core functions include: continuously clearing damaged neural tissue, plaques, and infectious substances from the central nervous system; expressing class II major histocompatibility complex and presenting antigens; and secreting cytokines to regulate the neuroinflammatory microenvironment. Overactivation of mouse microglial cells leads to the release of neurotoxic substances, which is closely associated with Parkinson's disease and Alzheimer's disease. In vitro-cultured mouse microglial cells serve as an important cell model for investigating neuroinflammation.

Handling Information

Instructions

1. Check all containers for leakage or breakage.
2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation.

Reagent Preparation

The culture period of Mouse Microglial Cells in vitro is limited; it is recommended to use the dedicated growth medium provided by Procell and follow the correct operation methods for cultivation to ensure the optimal culture state of these cells.

Product Name	Cat
Mouse Microglial Cell Complete Medium	CM-M110
12 mM lidocaine Dissociation Solution
Phosphate Buffer (PBS, 1 ×)	PB180327

Incubation Atmosphere

Air，95%；CO₂，5%

Temperature

37℃

Subcultivation Ratio

Non‑subculturable

Medium Renewal

Every 2 to 3 days

Population Doublings

Not recommended for expansion or long-term culture

Dissociation Reagent

Lidocaine (12 mM)

Freeze Medium

General Freezing Medium [PB180436]

Storage Conditions

For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150°C to −196°C. Storage at −80°C is restricted to short-term interim use only.

Documents

Manual

MSDS

Service Regulations

Feedback Form

Publications

Targeting microglial autophagic degradation of the NLRP3 inflammasome for identification of thonningianin A in Alzheimer’s disease

Journal: Inflammation and Regeneration (2022) IF: 10.4

DOI: 10.1186/s41232-022-00209-7

Product Cited: Mouse Microglia cells, Mouse Hippocampal Neuron cells, Mouse Microglia cells Complete Medium, Mouse Hippocampal Neuron cells Complete Medium

FAQs

Q:1 The color of cryopreserved cells varies, with some appearing pink and others yellowish. Does this variation affect their viability after thawing?
Answer
This phenomenon frequently occurs between different cryopreservation batches, and is associated with cell density, cryopreservation solution composition, and pH changes induced by temperature variations. This discoloration occurs only occasionally, and does not necessarily indicate compromised cell viability. We recommend attempting cell resuscitation first to assess their condition.
Q:2 How can I improve the survival rate of cryopreserved cells stored at -80°C?
Answer
When storing cryovials, place them in the innermost compartment of the refrigerator's freezer section; avoid placing them on the door shelves or near the refrigerator opening. Additionally, we recommend using refrigerators that are opened less frequently to minimize temperature fluctuations caused by opening and closing the door.
Q:3 How long can cells be preserved in a -80°C freezer?
Answer
The preservation duration of cells at -80°C depends on the operating frequency of the refrigerator and the temperature sensitivity of the cells themselves; therefore, there is no standardized storage period. It is recommended to transfer the cells into liquid nitrogen for long-term storage as early as possible.
Q:4 For frozen cells transported via dry ice, should they be stored at -80°C or in liquid nitrogen upon receipt?
Answer
It is recommended to immediately transfer the received frozen cells into liquid nitrogen for long-term storage; storage at -80℃ should only be used as a short-term interim solution. This is because at -80℃, cellular metabolism is significantly slowed but not completely halted. In addition, frequent opening and closing of the -80℃ freezer causes temperature fluctuations that reduce cell viability.
Q:5 My cells are growing very slowly. What could be the cause of this?
Answer
Please see some common reasons below, and solutions to the issue: - Growth medium is not correct: Use pre-warmed growth medium as recommended by the supplier. - Serum in the growth medium is of poor quality: Use serum from a different lot. and choose good quality serum to ensure nutrition. - Cells have been passaged too many times: Use healthy, low-passage number cells. - Cells were allowed to grow beyond confluency: Passage mammalian cells when they are in the log-phase before they reach confluency. - Culture is contaminated with mycoplasma: Discard cells, media, and reagents. Obtain new stocks of cells, and use them with fresh media and reagents.
Q:6 What factors can contribute to rapid cell death/culture failure?
Answer
There are a number of events that can contribute to this: 1. Incorrect CO2 levels:Monitor the level of CO2 manually with a Fyrite kit, available from Bacharach. Check if the manual readings concur with the readings displayed on the incubator. If the incubator has a trace readout, check the printout for fluctuations in CO2 level. Check the settings to ensure that CO2 levels are set at appropriate levels for your cell line (usually between 5 and 10%). Check line connections frequently for leaks. Avoid frequent opening and closing of incubator doors. 2. Temperature fluctuations in the incubator:Monitor the temperature of incubator with a good thermometer inside the incubator. 3. Amphotericin B or other preventive antibiotics/antimycotics are present at toxic concentrations:Use at recommended levels. 4. Humidity is incorrect:Check the water level in the water pan. Humidity is vital to appropriate gas exchange for many types of cells and media. 5. Incorrect osmotic pressure in medium:Check osmolality of complete medium. Most mammalian cells can tolerate an osmolality of 260 to 350 mOsm/kg. Additions of reagents such as HEPES and drugs may affect osmolality. 6. Contamination by microorganisms:Bacterial and fungal contaminations are usually easily visible; symptoms of mycoplasma contamination are more subtle, and careful monitoring of culture morphology and regular testing are necessary to detect this type of contamination. 7. Inappropriate medium is being used:Double-check that the medium used is appropriate for your cell type and culture application. For example, ensure that the medium being used for serum-free culture is actually designed for serum-free culture; make sure that appropriate selective drugs are used at appropriate levels; check the expiration dates for the reagents being used; and store medium at appropriate temperatures in the dark.