MSTO-211H

Name: MSTO-211H
Brand: Procell
SKU: CL-0163
Price: 500.0 USD
Availability: InStock

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Cat. No.: CL-0163

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CL-0163

$ 500.00

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1×10^6Cells/Vial×2Vials

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Product Introduction Documents Publications

Product Introduction

General information

Cell Name	MSTO-211H
Synonyms	MSTO-211 H;MSTO211H;MSTO-211;211H
Cellosaurus Accession	CVCL_1430
Organism	Human
Growth Properties	Adherent
Morphology	Fibroblast-like
Disease	lung cancer cells
Tissue	Lung
Age	62Y
Sex	Male

Handling information

Instructions	1. Check all containers for leakage or breakage. 2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation. 3. This cell line requires culture vessels coated with Poly-L-Lysine. This cell line exhibits weak adherence, so all manipulations must be carried out gently. Pre-warm the culture medium before medium exchange.
Complete Medium	RPMI-1640 [PM150110]+10% Nutrient+1% Supplement
Incubation Atmosphere	Air, 95%; CO₂, 5%
Temperature	37℃
Subcultivation Ratio	1:3-1:4
Medium Renewal	2 to 3 times per week
Dissociation Duration	2-3 min
Subculturing Procedure	1. Remove the culture medium from the T25 cell culture flask. 2. Add approximately 2 mL of PBS. Gently tilt the flask side to side until the PBS covers the entire bottom, then aspirate and discard the PBS. 3. Add 1 mL of 0.25% trypsin solution (containing EDTA). Gently tilt the flask side to side until the trypsin solution covers the entire bottom of the flask. 4. Incubate the cells at 37°C. Observe the cells under an inverted microscope and terminate digestion once the cells round up and detach. To avoid clumping, do not agitate the cells by tapping or shaking the flask during detachment. 5. Add 3 mL of complete culture medium to terminate digestion and disperse into a single cell suspension. 6. Collect the cell suspension and centrifuge at 1200 rpm (approximately 250 ×g) for 3 minutes. Carefully aspirate and discard the supernatant. 7. Add fresh complete culture medium, pipette gently several times to resuspend the cells, and seed them at the appropriate ratio into a new culture flask. Loosen the cap or use a vented cap for incubation.
Freeze Medium	General Freezing Medium [PB180436]
Storage Conditions	For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150°C to −196°C. Storage at −80°C is restricted to short-term interim use only.

Reference materials

Background	The MSTO-211H cell line was established in 1985 from the pleural effusion of a patient with biphasic pulmonary mesothelioma. The patient had received prior multidrug combination chemotherapy. MSTO-211H cells possess high-affinity EGF binding sites and express neuron-specific enolase (NSE) as well as the α and β subunits of human chorionic gonadotropin (HCG). The cells do not express detectable levels of L-dopa decarboxylase (DDC), bombesin, or neurotensin. MSTO-211H cells overexpress the c-myc proto-oncogene, and no gene rearrangements or amplifications have been observed. The cells show positive expression of v-src, v-abl, v-erbB, c-raf1, Ha-ras, Ki-ras, and N-ras. No expression of N-myc, L-myc, c-myb, c-fos, v-fes, v-fms, or v-sis oncogenes has been detected. The saturation density of MSTO-211H cells can reach 4 × 10⁵ cells/cm², at which point the cells detach from the culture surface.
Tumorigenic	Yes, tumors for med in approximately 20% of nude mice inoculated with MSTO-211H cells.
Biosafety Level	BSL-1