MT-4

Name: MT-4
Brand: Procell
SKU: CL-0655
Price: 420.0 USD
Availability: InStock

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Cat. No.: CL-0655

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CL-0655

$ 420.00

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1×10^6Cells/Vial×2Vials

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Product Introduction Documents Publications

Product Introduction

General information

Cell Name	MT-4
Cellosaurus Accession	CVCL_2632
Organism	Human
Growth Properties	Suspension
Morphology	Lymphoblast-like
Disease	lymphoma cells
Tissue	Peripheral blood
Age	<1D
Sex	Male

Handling information

Instructions	1. Check all containers for leakage or breakage. 2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation. 3. This cell line is a suspension cell line. Harvest the cell suspension by centrifugation; do not discard the culture medium directly.
Complete Medium	RPMI-1640 [PM150110]+10% Nutrient+1% Supplement
Incubation Atmosphere	Air, 95%; CO₂, 5%
Temperature	37℃
Subcultivation Ratio	3×10⁵-5×10⁵ cells/mL
Medium Renewal	2 to 3 times per week
Subculturing Procedure	Cultures can be maintained by either supplementing with fresh medium or exchanging medium via centrifugation. The recommended centrifugation conditions are 1200 rpm (approximately 250 × g) for 3 minutes.
Freeze Medium	General Freezing Medium [PB180436]
Storage Conditions	For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150°C to −196°C. Storage at −80°C is restricted to short-term interim use only.

Reference materials

Background

MT-4 is an adult T-cell leukaemia cell line derived from a 50-year-old Japanese male presenting with generalized lymphadenopathy and hepatosplenomegaly, established via co-culture of the patient’s peripheral leukocytes with male umbilical cord lymphocytes. The cells harbour HTLV-1 and support HIV propagation. Manipulation of this cell line requires Biosafety Level 3 containment facilities.