MUG-Chor1
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Cat. No.: CL-0993
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CL-0993
$ 500.00
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1×10^6Cells/Vial×2Vials
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Product Introduction
General information
| Cell Name | MUG-Chor1 |
| Synonyms | MUG-CHOR1;MUGCHOR1;Medical University of Graz-Chordoma 1 |
| Cellosaurus Accession | CVCL_9277 |
| Organism | Human |
| Growth Properties | Adherent |
| Morphology | Mesenchymal-like; long fusiform; irregular shape |
| Tissue | Bone |
| Age | 57Y |
| Sex | Female |
Handling information
| Instructions | 1. Check all containers for leakage or breakage. 2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation. 3. Centrifugation is required during dissociation and subculture; replace with new culture vessels. 4. This cell line proliferates slowly and exhibits density dependence; the subculture split ratio should not exceed 1:2. 5. A small number of floating cells and a small number of vacuoles are present during proliferation. |
| Complete Medium | IMDM [PM150510]+20% Nutrient+1% Supplement |
| Incubation Atmosphere | Air, 95%; CO₂, 5% |
| Temperature | 37℃ |
| Subcultivation Ratio | 1:2-1:3 |
| Medium Renewal | 2 to 3 times per week |
| Dissociation Duration | 3-4 min |
| Subculturing Procedure | 1. Remove the culture medium from the T25 cell culture flask. 2. Add approximately 2 mL of PBS. Gently tilt the flask side to side until the PBS covers the entire bottom, then aspirate and discard the PBS. 3. Add 1 mL of 0.25% trypsin solution (containing EDTA). Gently tilt the flask side to side until the trypsin solution covers the entire bottom of the flask. 4. Incubate the cells at 37°C. Observe the cells under an inverted microscope and terminate digestion once the cells round up and detach. To avoid clumping, do not agitate the cells by tapping or shaking the flask during detachment. 5. Add 3 mL of complete culture medium to terminate digestion and disperse into a single cell suspension. 6. Collect the cell suspension and centrifuge at 1200 rpm (approximately 250 ×g) for 3 minutes. Carefully aspirate and discard the supernatant. 7. Add fresh complete culture medium, pipette gently several times to resuspend the cells, and seed them at the appropriate ratio into a new culture flask. Loosen the cap or use a vented cap for incubation. |
| Freeze Medium | 90% FBS+10% DMSO |
| Storage Conditions | For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150°C to −196°C. Storage at −80°C is restricted to short-term interim use only. |
Reference materials
| Background | MUG-Chor1 is a mesenchymal-like cell line established in 2009 from the sacrum of a 57-year-old White female patient with chordoma. As a rare, indolent neoplasm, chordoma is recapitulated by this slowly proliferating cell line. MUG-Chor1 displays heterogeneous morphology with physaliferous cells and mucinous intercellular components, hallmark features of chordoma. These cells harbor amplification of the transcription factor T (Brachyury)—the most specific biomarker for chordoma—as well as PTEN gene deletion. |
| Biosafety Level | BSL-1 |
MSDS
