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Product Introduction
| Cell Name | NB4 |
| Synonyms | NB-4;NB.4 |
| Cellosaurus Accession | CVCL_0005 |
| Organism | Human |
| Growth Properties | Suspension |
| Morphology | Lymphoblast-like |
| Disease | leukemia cells |
| Tissue | Bone marrow |
| Age | 23Y |
| Sex | Female |
| Instructions | 1. Check all containers for leakage or breakage. 2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation. |
| Complete Medium | RPMI-1640 [PM150110]+10% Nutrient+1% Supplement |
| Incubation Atmosphere | Air, 95%; CO₂, 5% |
| Temperature | 37℃ |
| Subcultivation Ratio | 1:2-1:3 |
| Medium Renewal | 2 to 3 times per week |
| Subculturing Procedure | Cultures can be maintained by either supplementing with fresh medium or exchanging medium via centrifugation. The recommended centrifugation conditions are 1200 rpm (approximately 250 × g) for 3 minutes. |
| Freeze Medium | General Freezing Medium [PB180436] |
| Storage Conditions | For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150°C to −196°C. Storage at −80°C is restricted to short-term interim use only. |
| Background | The NB-4 cell line was established in 1989 from the bone marrow of a patient with acute promyelocytic leukemia (APL; AML FAB M3) in second relapse. |
| Biosafety Level | BSL-1 |
Documents
MSDSPublications
Journal: Molecular Cancer (2024) IF: 27.7
DOI: 10.1186/s12943-024-02013-y
Product Cited: HL-60 Cells Line, THP-1 Cells Line, RPMI-1640 Complete Medium (10), DMEM (High glucose) Complete Medium (10), IMDM Complete Medium (10), 293T [HEK-293T] Cells Line, NB4 Cells Line
Journal: FREE RADICAL BIOLOGY AND MEDICINE (2024) IF: 7.1
DOI: 10.1016/j.freeradbiomed.2024.06.014
Product Cited: U-937 Cells Line, Jurkat, Clone E6-1 Cells Line, NB4 Cells Line, BxPC-3 Cells Line, SU-DHL-4 Cells Line, Caov-3 Cells Line, HuH-7 Cells Line, HT-1080 [HT1080] Cells Line
Journal: MOLECULAR CARCINOGENESIS (2024) IF: 4.6
DOI: 10.1002/mc.23715
Product Cited: NB4 Cells Line, KG-1 Cells Line
Journal: GENE (2024) IF: 3.5
DOI: 10.1016/j.gene.2024.148443
Product Cited: NB4 Cells Line
Journal: Oncology Letters (2024) IF: 2.9
Product Cited: NOMO-1 Cells Line, MOLM-13 Cells Line, NB4 Cells Line, BALL-1 Cells Line, NALM-6 Cells Line, SUP-B15 Cells Line
