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NB4

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Cat. No.: CL-0676 Publications(20)
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CL-0676

$ 460.00

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1×10^6Cells/Vial×2Vials

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Product Introduction Documents Publications

Product Introduction

General information
Cell Name NB4
Synonyms NB-4;NB.4
Cellosaurus Accession CVCL_0005
Organism Human
Growth Properties Suspension
Morphology Lymphoblast-like
Disease leukemia cells
Tissue Bone marrow
Age 23Y
Sex Female
Instructions 1. Check all containers for leakage or breakage.
2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation.
Complete Medium RPMI-1640 [PM150110]+10% Nutrient+1% Supplement
Incubation Atmosphere Air, 95%; CO₂, 5%
Temperature 37℃
Subcultivation Ratio 1:2-1:3
Medium Renewal 2 to 3 times per week
Subculturing Procedure Cultures can be maintained by either supplementing with fresh medium or exchanging medium via centrifugation. The recommended centrifugation conditions are 1200 rpm (approximately 250 × g) for 3 minutes.
Freeze Medium General Freezing Medium [PB180436]
Storage Conditions For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150°C to −196°C. Storage at −80°C is restricted to short-term interim use only.
Background The NB-4 cell line was established in 1989 from the bone marrow of a patient with acute promyelocytic leukemia (APL; AML FAB M3) in second relapse.
Biosafety Level BSL-1

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