NCCIT

Name: NCCIT
Brand: Procell
SKU: CL-0639
Price: 500.0 USD
Availability: InStock

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Cat. No.: CL-0639

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CL-0639

$ 500.00

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1×10^6Cells/Vial×2Vials

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Product Introduction Documents Publications

Product Introduction

General information

Cell Name	NCCIT
Synonyms	NCC-IT
Cellosaurus Accession	CVCL_1451
Organism	Human
Growth Properties	Adherent
Morphology	Epithelial-like
Tissue	Mediastinum
Age	24Y
Sex	Male

Handling information

Instructions	1. Check all containers for leakage or breakage. 2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation. 3. This cell line is relatively sensitive to temperature; it is recommended to pre-warm the culture medium before handling cells. 4. Meanwhile, this cell line has relatively high requirements for serum and is significantly affected by serum. 5. A certain amount of floating cells is observed during culture. The culture medium tends to turn yellow at both high and low cell densities. Floating cell debris is present during cell growth. 6. Dissociation time during subculture is approximately 3 minutes. Subculture at a 1:2 split ratio; cells can be subcultured again after approximately 3 days. Centrifugation followed by cell resuspension is recommended after dissociation. 7. Replacing with new culture vessels during subculture reduces non-adherence. It is recommended to coat culture vessels with Poly-L-Lysine.
Complete Medium	RPMI-1640 [PM150110]+10% Nutrient+1% Supplement
Incubation Atmosphere	Air, 95%; CO₂, 5%
Temperature	37℃
Subcultivation Ratio	1:2-1:3
Medium Renewal	2 to 3 times per week
Dissociation Duration	3 min
Subculturing Procedure	1. Remove the culture medium from the T25 cell culture flask. 2. Add approximately 2 mL of PBS. Gently tilt the flask side to side until the PBS covers the entire bottom, then aspirate and discard the PBS. 3. Add 1 mL of 0.25% trypsin solution (containing EDTA). Gently tilt the flask side to side until the trypsin solution covers the entire bottom of the flask. 4. Incubate the cells at 37°C. Observe the cells under an inverted microscope and terminate digestion once the cells round up and detach. To avoid clumping, do not agitate the cells by tapping or shaking the flask during detachment. 5. Add 3 mL of complete culture medium to terminate digestion and disperse into a single cell suspension. 6. Collect the cell suspension and centrifuge at 1200 rpm (approximately 250 ×g) for 3 minutes. Carefully aspirate and discard the supernatant. 7. Add fresh complete culture medium, pipette gently several times to resuspend the cells, and seed them at the appropriate ratio into a new culture flask. Loosen the cap or use a vented cap for incubation.
Freeze Medium	General Freezing Medium [PB180436]
Storage Conditions	For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150°C to −196°C. Storage at −80°C is restricted to short-term interim use only.

Reference materials

Background	NCCIT was established by Shinichi Teshima (National Cancer Institute, Tokyo, Japan) in 1985 from a mediastinal mixed germ cell tumor.
Tumorigenic	Yes, Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 10⁷ cells.
Biosafety Level	BSL-1