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CL-0815
$ 600.00
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1×10^6Cells/Vial×2Vials
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Product Introduction
General information
| Cell Name | NCI-BL2009 |
| Synonyms | BL2009 |
| Cellosaurus Accession | CVCL_2645 |
| Organism | Human |
| Growth Properties | Suspension |
| Morphology | Lymphoblast-like |
| Tissue | Peripheral blood |
| Age | 68Y |
| Sex | Female |
Handling information
| Instructions | 1. Check all containers for leakage or breakage. 2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation. |
| Complete Medium | RPMI-1640 [PM150110]+10% Nutrient+1% Supplement |
| Incubation Atmosphere | Air, 95%; CO₂, 5% |
| Temperature | 37℃ |
| Subcultivation Ratio | 1:2-1:4 |
| Medium Renewal | 2 to 3 times per week |
| Subculturing Procedure | Cultures can be maintained by either supplementing with fresh medium or exchanging medium via centrifugation. The recommended centrifugation conditions are 1200 rpm (approximately 250 × g) for 3 minutes. |
| Freeze Medium | General Freezing Medium [PB180436] |
| Storage Conditions | For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150°C to −196°C. Storage at −80°C is restricted to short-term interim use only. |
Reference materials
| Background | NCI-BL2009 are B lymphoblasts that were isolated from the peripheral blood of a normal 68-year-old. |
| Biosafety Level | BSL-2 |
Documents
MSDSPublications
Unravelling NK cell subset dynamics and specific gene signatures post-ibrutinib therapy in chronic lymphocytic leukaemia via single-cell transcriptomics
Journal: BMC CANCER (2025) IF: 3.4
DOI: 10.1186/s12885-025-14166-0
Product Cited: NCI-BL2009 Cells Line
EBV?+?B cell-derived exosomes promote EBV-associated T/NK-cell lymphoproliferative disease immune evasion by STAT3/IL-10/PD-L1 pathway
Journal: IMMUNOLOGIC RESEARCH (2024) IF: 3.3
DOI: 10.1007/s12026-024-09531-3
Product Cited: CA46 Cells Line, NCI-BL2009 Cells Line, HuT 78 Cells Line, H9 [derivative of HuT 78] Cells Line
