NCI-H1299

Name: NCI-H1299
Brand: Procell
SKU: CL-0165
Price: 420.0 USD
Availability: InStock

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Cat. No.: CL-0165

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CL-0165

$ 420.00

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1×10^6Cells/Vial×2Vials

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Product Introduction Documents Publications

Product Introduction

General information

Cell Name	NCI-H1299
Synonyms	H1299;H-1299;NCIH1299
Cellosaurus Accession	CVCL_0060
Organism	Human
Growth Properties	Adherent
Morphology	Epithelial-like
Disease	lung cancer cells
Tissue	Lung; derived from metastatic site: lymph node
Age	43Y
Sex	Male

Handling information

Instructions	1. Check all containers for leakage or breakage. 2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation.
Complete Medium	RPMI-1640 [PM150110]+10% Nutrient+1% Supplement
Incubation Atmosphere	Air, 95%; CO₂, 5%
Temperature	37℃
Subcultivation Ratio	1:3-1:6
Medium Renewal	2 to 3 times per week
Dissociation Duration	2-3 min
Subculturing Procedure	1. Remove the culture medium from the T25 cell culture flask. 2. Add approximately 2 mL of PBS. Gently tilt the flask side to side until the PBS covers the entire bottom, then aspirate and discard the PBS. 3. Add 1 mL of 0.25% trypsin solution (containing EDTA). Gently tilt the flask side to side until the trypsin solution covers the entire bottom of the flask. 4. Incubate the cells at 37°C. Observe the cells under an inverted microscope and terminate digestion once the cells round up and detach. To avoid clumping, do not agitate the cells by tapping or shaking the flask during detachment. 5. Add 3 mL of complete culture medium to terminate digestion and disperse into a single cell suspension. 6. Collect the cell suspension and centrifuge at 1200 rpm (approximately 250 ×g) for 3 minutes. Carefully aspirate and discard the supernatant. 7. Add fresh complete culture medium, pipette gently several times to resuspend the cells, and seed them at the appropriate ratio into a new culture flask. Loosen the cap or use a vented cap for incubation.
Freeze Medium	Freezing Medium (Serum-free & animal origin-free) [PB180438]
Storage Conditions	For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150°C to −196°C. Storage at −80°C is restricted to short-term interim use only.

Reference materials

Background	NCI-H1299 cells were derived from a lymph node metastasis of a patient who had received prior radiation therapy. The cells harbor a homozygous partial deletion of the p53 gene and lack p53 protein expression. NCI-H1299 cells can synthesize neuromedin B (NMB) at 0.1 pmol/mg protein but do not produce gastrin-releasing peptide (GRP).
Gene Expression	These cells stain positive for keratin and vimentin but are negative for neurofilament triplet protein. neuromedin B, The cells have a homozygous partial deletion of the p53 protein, and lack expression of p53 protein. The cells produce neuromedin B.
Biosafety Level	BSL-1