NCI-H146 [H146]

Name: NCI-H146 [H146]
Brand: Procell
SKU: CL-0693
Price: 460.0 USD
Availability: InStock

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Cat. No.: CL-0693

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CL-0693

$ 460.00

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1×10^6Cells/Vial×2Vials

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Product Introduction Documents Publications

Product Introduction

General information

Cell Name	NCI-H146 [H146]
Synonyms	H146;H-146;NCIH146
Cellosaurus Accession	CVCL_1473
Organism	Human
Growth Properties	Suspension with clustering
Morphology	Lymphoblast-like
Disease	lung cancer cells
Tissue	Lung
Age	59Y
Sex	Male

Handling information

Instructions	1. Check all containers for leakage or breakage. 2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation. 3. This cell line is a suspension cell line. Harvest the cell suspension by centrifugation; do not discard the culture medium directly.
Complete Medium	RPMI-1640 [PM150110]+10% Nutrient+1% Supplement
Incubation Atmosphere	Air, 95%; CO₂, 5%
Temperature	37℃
Subcultivation Ratio	1:2-1:3
Medium Renewal	2 to 3 times per week
Subculturing Procedure	Cultures can be maintained by either supplementing with fresh medium or exchanging medium via centrifugation. The recommended centrifugation conditions are 1200 rpm (approximately 250 × g) for 3 minutes.
Freeze Medium	General Freezing Medium [PB180436]
Storage Conditions	For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150°C to −196°C. Storage at −80°C is restricted to short-term interim use only.

Reference materials

Background

NCI-H146 [H146] is a human small cell lung cancer cell line established from pleural fluid collected from a SCLC patient. NCI-H146 [H146] cells overexpress four classic biochemical markers specific to small cell lung carcinoma: neuron-specific enolase, brain-type creatine kinase isoenzyme, L-dopa decarboxylase, and bombesin-like immunoreactivity. Immunocytochemical staining shows positive signals for keratin and vimentin, whereas neurofilament triplet protein expression is absent. At the transcriptional level, NCI-H146 [H146] exhibits relatively abundant c-myc mRNA expression, though no genomic amplification of the c-myc gene is detected in this cell line.

Gene Expression

vasopressin negative; oxytocin negative; gastrin releasing peptide negative; vimentin positive by immunostaining; keratin positive by immunostaining; neurofilament triplet protein negative; c-myb mRNA; v-fms mRNA; Ha-ras mRNA; Ki-ras mRNA; N-ras mRNA; c-raf 1 mRNA; myc+; myb+; raf+; ras+; fms+; fes -