NCI-H187
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Cat. No.: CL-0966
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CL-0966
$ 500.00
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1×10^6Cells/Vial×2Vials
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Product Introduction
General information
| Cell Name | NCI-H187 |
| Synonyms | H187;H-187;NCIH187 |
| Cellosaurus Accession | CVCL_1501 |
| Organism | Human |
| Growth Properties | Suspension with multicellular aggregation |
| Morphology | Round; growing in clusters |
| Disease | lung cancer cells |
| Tissue | Lung; derived from metastatic site: pleural effusion |
| Age | 47Y |
| Sex | Male |
Handling information
| Instructions | 1. Check all containers for leakage or breakage. 2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation. |
| Complete Medium | RPMI-1640 [PM150110]+10% Nutrient+1% Supplement |
| Incubation Atmosphere | Air, 95%; CO₂, 5% |
| Temperature | 37℃ |
| Medium Renewal | 2 to 3 times per week |
| Subculturing Procedure | Cultures can be maintained by either supplementing with fresh medium or exchanging medium via centrifugation. The recommended centrifugation conditions are 1200 rpm (approximately 250 × g) for 3 minutes. |
| Freeze Medium | General Freezing Medium [PB180436] |
| Storage Conditions | For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150 °C to −196 °C. Storage at −80 °C is restricted to short-term interim use only. |
Reference materials
| Background | NCI-H187 was established from cells isolated from a pleural effusion of a patient prior to treatment. |
| Tumorigenic | 裸鼠成瘤 |
| Biosafety Level | BSL-1 |
MSDS
