NCI-H209 [H209]

Name: NCI-H209 [H209]
Brand: Procell
SKU: CL-0393
Price: 500.0 USD
Availability: InStock

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Cat. No.: CL-0393

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1×10^6Cells/Vial×2Vials

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Product Introduction Documents Publications

General information

Cell Name	NCI-H209 [H209]
Synonyms	H209;H-209;NCIH209
Cellosaurus Accession	CVCL_1525
Organism	Human
Growth Properties	Suspension with multicellular aggregation
Morphology	Round; growing in clusters
Disease	lung cancer cells
Tissue	Lung; derived from metastatic site: bone marrow
Age	55Y
Sex	Male

Handling information

Instructions	1. Check all containers for leakage or breakage. 2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation. 3. This cell line is a suspension cell line. Harvest the cell suspension by centrifugation; do not discard the culture medium directly.
Complete Medium	RPMI-1640 [PM150110]+20% Nutrient+1% Supplement
Incubation Atmosphere	Air, 95%; CO₂, 5%
Temperature	37℃
Subcultivation Ratio	1:2-1:3
Medium Renewal	2 to 3 times per week
Subculturing Procedure	Cultures can be maintained by either supplementing with fresh medium or exchanging medium via centrifugation. The recommended centrifugation conditions are 1200 rpm (approximately 250 × g) for 3 minutes.
Freeze Medium	90% FBS+10% DMSO
Storage Conditions	For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150°C to −196°C. Storage at −80°C is restricted to short-term interim use only.

Reference materials

Background	NCI-H209 [H209] cells were isolated and established in 1979 by A.F. Gazdar and colleagues from a bone marrow metastasis of a patient with small cell lung cancer. The bone marrow specimen was obtained prior to the patient's treatment. NCI-H209 cells are a classic small cell lung cancer cell line expressing high levels of four biochemical markers: neuron-specific enolase, brain-type creatine kinase, L-dopa decarboxylase, and bombesin-like immunoreactivity. The c-myc DNA sequence is not amplified, and no gross structural DNA abnormalities have been found. NCI-H209 cells synthesize p53 mRNA at levels comparable to those in normal lung tissue. NCI-H209 cells grow in suspension as aggregates; only cells within the aggregates are viable, and viability cannot be estimated. Typically, the culture medium contains a large amount of cellular debris.
Tumorigenic	Yes, forms transplantable tumors with typical SCLC histology in nude mice.
Biosafety Level	BSL-1