NCI-H2122
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Cat. No.: CL-0861
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CL-0861
$ 500.00
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1×10^6Cells/Vial×2Vials
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Product Introduction
General information
| Cell Name | NCI-H2122 |
| Synonyms | H2122;H-2122;NCIH2122 |
| Cellosaurus Accession | CVCL_1531 |
| Organism | Human |
| Growth Properties | Semi-adherent and semi-suspension |
| Morphology | Lymphoblast-like; round; single cells; occasional aggregation |
| Disease | lung cancer cells |
| Tissue | Lung; derived from metastatic site: pleural effusion |
| Age | 46Y |
| Sex | Female |
Handling information
| Instructions | 1. Check all containers for leakage or breakage. 2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation. 3. Semi-adherent and semi-suspension. Collect the culture medium and centrifuge. |
| Complete Medium | RPMI-1640 [PM150110]+10% Nutrient+1% Supplement |
| Incubation Atmosphere | Air, 95%; CO₂, 5% |
| Temperature | 37℃ |
| Subcultivation Ratio | 1:3-1:4 |
| Medium Renewal | 2 to 3 times per week |
| Dissociation Duration | 2-4 min |
| Subculturing Procedure | 1. This cell line displays mixed adherent and suspended phenotypes. Suspended cells remain viable. Transfer the single-cell suspension into a centrifuge tube and pellet the cells via centrifugation at 1200 × g (approximately 250 × g). 2. Weakly adherent cells can be dispersed by gentle pipetting to form a homogeneous single-cell suspension. 3. For firmly adherent cells, rinse with PBS, then add 1–2 mL of 0.25% Trypsin-EDTA to the culture flask. Incubate at 37°C. Once the cells round up and detach from the vessel surface, add 4–6 mL of complete medium to terminate digestion. Gently pipette to generate a single-cell suspension, then harvest cells by centrifugation. 4. Mix all harvested suspended and adherent cell populations, then seed the cells into fresh culture vessels at the recommended split ratio. |
| Freeze Medium | General Freezing Medium [PB180436] |
| Storage Conditions | For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150°C to −196°C. Storage at −80°C is restricted to short-term interim use only. |
Reference materials
| Background | NCI-H2122 cells were isolated in 1989 from a pleural effusion metastasis of lung adenocarcinoma obtained from a 46-year-old female patient with a history of smoking. |
| Biosafety Level | BSL-1 |
MSDS
