NCI-H2171
Copy the product info.
Cat. No.: CL-1094
-
-
-
- +2
CL-1094
$ 500.00
Select a size
1×10^6Cells/Vial×2Vials
Quantity
-
+
In stock
InquireFor order requirements and technical needs, please Contact Us
Product Introduction
General information
| Cell Name | NCI-H2171 |
| Synonyms | H2171;H-2171;NCIH2171 |
| Cellosaurus Accession | CVCL_1536 |
| Organism | Human |
| Growth Properties | Suspension |
| Morphology | Lymphoblast-like |
| Tissue | Lung; derived from metastatic site: pleural effusion |
| Age | 50Y |
| Sex | Male |
Handling information
| Instructions | 1. Check all containers for leakage or breakage. 2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation. 3. Dark granules appear when cell condition is poor during culture; a small amount of dark granules remains after cell condition is adjusted. |
| Complete Medium | DMEM/F12 [PM150312]+0.5% Supplement1+10 nM Supplement2+10 nM Supplement3+10% Nutrient+1% Supplement4 |
| Incubation Atmosphere | Air, 95%; CO₂, 5% |
| Temperature | 37℃ |
| Subcultivation Ratio | 1:2-1:4 |
| Medium Renewal | 2 to 3 times per week |
| Subculturing Procedure | Cultures can be maintained by either supplementing with fresh medium or exchanging medium via centrifugation. The recommended centrifugation conditions are 1200 rpm (approximately 250 × g) for 3 minutes. |
| Freeze Medium | General Freezing Medium [PB180436] |
| Storage Conditions | For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150°C to −196°C. Storage at −80°C is restricted to short-term interim use only. |
Reference materials
| Background | NCI-H2171 are lymphoblasts exhibiting floating clusters morphology that was isolated from the lungs of a 50-year-old, White male patient with stage E small cell lung cancer: carcinoma. |
| Biosafety Level | BSL-1 |
MSDS
