NCI-H292

Name: NCI-H292
Brand: Procell
SKU: CL-0167
Price: 420.0 USD
Availability: InStock

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Cat. No.: CL-0167

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CL-0167

$ 420.00

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1×10^6Cells/Vial×2Vials

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Product Introduction Documents Publications

Product Introduction

General information

Cell Name	NCI-H292
Synonyms	H292;H-292;NCI-HUT-292;Hut292;NCIH292
Cellosaurus Accession	CVCL_0455
Organism	Human
Growth Properties	Adherent
Morphology	Epithelial-like
Disease	lung cancer cells
Tissue	Lung; derived from metastatic site: lymph node
Age	32Y
Sex	Female

Handling information

Instructions	1. Check all containers for leakage or breakage. 2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation. 3. A small number of cytoplasmic vacuoles may be observed during cell cultivation.
Complete Medium	RPMI-1640 [PM150110]+10% Nutrient+1% Supplement
Incubation Atmosphere	Air, 95%; CO₂, 5%
Temperature	37℃
Subcultivation Ratio	1:2-1:4
Medium Renewal	2 to 3 times per week
Dissociation Duration	2-3 min
Subculturing Procedure	1. Remove the culture medium from the T25 cell culture flask. 2. Add approximately 2 mL of PBS. Gently tilt the flask side to side until the PBS covers the entire bottom, then aspirate and discard the PBS. 3. Add 1 mL of 0.25% trypsin solution (containing EDTA). Gently tilt the flask side to side until the trypsin solution covers the entire bottom of the flask. 4. Incubate the cells at 37°C. Observe the cells under an inverted microscope and terminate digestion once the cells round up and detach. To avoid clumping, do not agitate the cells by tapping or shaking the flask during detachment. 5. Add 3 mL of complete culture medium to terminate digestion and disperse into a single cell suspension. 6. Collect the cell suspension and centrifuge at 1200 rpm (approximately 250 ×g) for 3 minutes. Carefully aspirate and discard the supernatant. 7. Add fresh complete culture medium, pipette gently several times to resuspend the cells, and seed them at the appropriate ratio into a new culture flask. Loosen the cap or use a vented cap for incubation.
Freeze Medium	Freezing Medium (Serum-free & animal origin-free) [PB180438]
Storage Conditions	For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150°C to −196°C. Storage at −80°C is restricted to short-term interim use only.

Reference materials

Background	NCI-H292 cells were derived from a lymph node metastasis of a pulmonary mucoepidermoid carcinoma. The cells were initially isolated using a defined medium and subsequently cultured in serum-containing medium. Under culture conditions, NCI-H292 cells retain their mucoepidermoid characteristics, as evidenced by their ultrastructure and the expression of multiple squamous differentiation markers. NCI-H292 cells support the growth of HBV and are negative for L-DOPA decarboxylase. These cells can serve as a screening model for transfecting human subgenomic fragments to study the role of HBV and its individual genes in the pathogenesis of viral hepatitis and liver cancer. NCI-H292 cells stain positively for keratin, vimentin, and mucicarmine, but are negative for neurofilament triplet protein.
Tumorigenic	Yes, in nude mice; tumors histologically resemble the original biopsy specimen and retain mucoepidermoid features.
Gene Expression	keratin; vimentin,The cells retain their mucoepidermoid characteristics in culture as determined by their ultrastructure and expression of multiple markers of squamous differentiation. TANK cells show reactivity with human alloantisera specific for the HLA A1, A3, A10, A19, B12, B17, B22 and B40 cross-reactive groups.
Biosafety Level	BSL-1