NCI-H446-GFP
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Cat. No.: CL-1128
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CL-1128
$ 720.00
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1×10^6Cells/Vial×2Vials
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Product Introduction
General information
| Cell Name | NCI-H446-GFP |
| Organism | Human |
| Growth Properties | Semi-adherent and semi-suspension |
| Morphology | Epithelial-like |
| Disease | lung cancer cells |
| Tissue | Lung; derived from metastatic site: pleural effusion |
| Age | 61Y |
| Sex | Male |
Handling information
| Instructions | 1. Check all containers for leakage or breakage. 2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation. |
| Complete Medium | RPMI-1640 [PM150110]+10% Nutrient+1% Supplement |
| Incubation Atmosphere | Air, 95%; CO₂, 5% |
| Temperature | 37℃ |
| Subcultivation Ratio | 1:2-1:4 |
| Medium Renewal | 2 to 3 times per week |
| Dissociation Duration | 1-2 min |
| Subculturing Procedure | 1. This cell line displays mixed adherent and suspended phenotypes. Although suspended cells remain viable, their density is low and they may be aspirated and discarded directly. 2. Rinse the adherent cells with PBS, then add 1–2 mL of 0.25% Trypsin-EDTA to the culture flask. Incubate at 37°C. Once the cells round up and detach from the vessel surface, add 4–6 mL of complete medium to terminate digestion. Gently pipette to generate a single-cell suspension, then harvest cells by centrifugation. 3. Seed the cells into fresh culture vessels at the recommended split ratio. |
| Freeze Medium | Freezing Medium (Serum-free & animal origin-free) [PB180438] |
| Storage Conditions | For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150°C to −196°C. Storage at −80°C is restricted to short-term interim use only. |
Reference materials
| Background | NCI‑H446 cells were established from the pleural effusion of a patient with small cell lung cancer. The original morphology of NCI-H446 cells does not display characteristic features of small cell lung cancer. This cell line is a biochemical and morphological variant of small cell lung cancer, expressing neuron-specific enolase and the brain isoenzyme of creatine kinase. L-DOPA decarboxylase, bombesin, vasopressin, oxytocin, and gastrin-releasing peptide were undetectable in NCI-H446 cells. The c-myc DNA sequence is amplified approximately 20-fold, and c-myc RNA levels are increased 15-fold relative to normal cells. The initial culture medium consisted of RPMI-1640 supplemented with 5% fetal bovine serum, 10 nM hydrocortisone, 0.005 mg/mL insulin, 0.01 mg/mL transferrin, 10 nM 17β-estradiol, and 30 nM sodium selenite.. NCI‑H446‑GFP cells were constructed from NCI-H446 cells by lentiviral transduction and is a stable cell line capable of long‑term, high‑level GFP expression. This cell line exhibits low passage, high viability, and good condition, making it suitable for assays such as flow cytometry and fluorescence imaging. Selection drug concentration: Puro = 1.0 μg/mL. |
| Tumorigenic | Yes, in nude mice (The cells form transplantable tumors with non-typical SCLC histology). |
| Biosafety Level | BSL-2 |
MSDS
