NCI-H446 [H446]

Name: NCI-H446 [H446]
Brand: Procell
SKU: CL-0401
Price: 500.0 USD
Availability: InStock

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Cat. No.: CL-0401

Publications(13)

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1×10^6Cells/Vial×2Vials

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Product Introduction Documents Publications

General information

Cell Name	NCI-H446 [H446]
Synonyms	H446;H-446;NCI-446;NCIH446
Cellosaurus Accession	CVCL_1562
Organism	Human
Growth Properties	Semi-adherent and semi-suspension
Morphology	Epithelial-like
Disease	lung cancer cells
Tissue	Lung; derived from metastatic site: pleural effusion
Age	61Y
Sex	Male

Handling information

Instructions	1. Check all containers for leakage or breakage. 2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation.
Complete Medium	RPMI-1640 [PM150110]+10% Nutrient+1% Supplement
Incubation Atmosphere	Air, 95%; CO₂, 5%
Temperature	37℃
Subcultivation Ratio	1:2-1:4
Medium Renewal	2 to 3 times per week
Dissociation Duration	1-2 min
Subculturing Procedure	1. This cell line displays mixed adherent and suspended phenotypes. Although suspended cells remain viable, their density is low and they may be aspirated and discarded directly. 2. Rinse the adherent cells with PBS, then add 1–2 mL of 0.25% Trypsin-EDTA to the culture flask. Incubate at 37°C. Once the cells round up and detach from the vessel surface, add 4–6 mL of complete medium to terminate digestion. Gently pipette to generate a single-cell suspension, then harvest cells by centrifugation. 3. Seed the cells into fresh culture vessels at the recommended split ratio.
Freeze Medium	Freezing Medium (Serum-free & animal origin-free) [PB180438]
Storage Conditions	For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150°C to −196°C. Storage at −80°C is restricted to short-term interim use only.

Reference materials

Background	NCI-H446 [H446] cells were established from the pleural effusion of a patient with small cell lung cancer (SCLC). The native morphology of NCI-H446 cells does not exhibit the characteristic features of SCLC. This cell line is a biochemical and morphological variant of SCLC, expressing neuron-specific enolase and the brain isoenzyme of creatine kinase. L-DOPA decarboxylase, bombesin, vasopressin, oxytocin, and gastrin-releasing peptide are undetectable in NCI-H446 cells. The <i>c-MYC</i> DNA sequence is amplified ~ 20-fold, and <i>c-MYC</i> RNA levels are increased 15-fold relative to normal cells. The initial growth medium consisted of RPMI-1640 medium supplemented with 5% fetal bovine serum (fetal bovine serum (FBS)), 10 nM hydrocortisone, 0.005 mg/mL insulin, 0.01 mg/mL transferrin, 10 nM 17β-estradiol, and 30 nM sodium selenite.
Tumorigenic	Yes, in nude mice (The cells form transplantable tumors with non-typical SCLC histology).
Biosafety Level	BSL-1